동종이식내성의 유치에 관한 실험

Abstract

Since Billinghams success of inducing acquired toleranee of homologous skin graft by prenatal injection of spleen cells in mice Woodruff et al and Egdahl et al also produced tolerance in rats injected postnatally with spleen cells, while Kim successfully induced tolerance by postnatal injection of refrigerated whole blood as well as spleen cells. This study was performed to see whether postpartum mduction of tolerance to homologous skin in dogs which are rather high phylogenetically can be produced by injecting whole blood as well as spleen cells. Twenty puppies from 3 Mongrel mother dogs were used as recipients and 3 male adult Shepherd dogs were used as donors. As the control animals an optional unrelated Shep· hered dog or Mongrel dog, mother dogs and the same litter siblings were used. Each recipient was injected intraperitoneally with 3. Occ-5.Occ of spleen cell suspension or whole blood from prospective donors on the day of birth and at the age of 40-56 days was grafted with skin of 2. 0 em. x 3. 0 em. from corresponding cell donors , optional dogs and siblings. Of the 12 grafted dogs 2 animals of 2 litters injected with spleen cells showed prolonged survival of donor skin(23-28 days after grafting), followed by the chronic rejection; whereas the skin from unrelated donor and mother dog sloughed within the first postoperative week and the reciprocal skin grafts of the same Jitter ap-imals were rejected within 10 days with the typical pattern of homograft rejection. In animals of 2 litters injected with whole blood grafts from corresponding cell donors also survived for 22•29 days after grafting while the control grafts sloughed from 7•10 days postoperatively