구강점막세포의 휴지기핵에서의 Y형광체에 관한 연구

Abstract

To evaluate the rate of appearance of Yfluorescent body in interphase nuclei of normal human buccal smears, 50 coded slides (25 males and 25 females) were obtained from the medical student volunteers, stained with either one of the 3 staining solutions: quinacrine mustard buffer;quinacrine dihydrochloride water and quinacrine dihydrochloride buffer. The stained slides were blindly scorered with a count of 100 nuclei. Although four slides were found to be misclassified, generally prediction of the sex of the individual from whom the specimen was taken was satisfactory. The mistake in classification took place mainly because of poor technical quality of the smears, which could be predicted even before decoding process. According to our experience this technique seems to be very useful in sex determination of the interphase nuclei , being complementary to the conventional sex chromatin body study.