급성 일산화탄소 중독이 백서소뇌 Purkinje 세포에 미치는 영향에 관한 세포병리학적 연구
Cytopathologic Study of Cerebellar Purkinje Cells on the Effect of Acute Carbon Monoxide Intoxication in Rats

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신동휘; 송계용; 함의근; 심보성
Issue Date
서울대학교 의과대학
Seoul J Med 1981;22(1):46-66
An experimental study was performed to observe
the cytopathologic effects of acute carbon monoxide
intoxication in lapse on the cerebellar Purkinje cells
。f rats by means of cytologic, enzyme cytochemical
methods using light and electron microscope attem'
pting to explore the pathogenesis related to ultrastr
uctural features and enzyme cytochemistry.
Experimental animals(125 Wister rats , 300-350gm.)
were devided into control and exposure groups which
were exposed by 0.5%CO for 45 min. And thereafter
left to fresh air in 15 minutes through 30 days past
to acute carbon monoxide intoxication. Cytopathologic
investigation around the stainability, changes of Nissl substances and ultrastructural study on Purl‘inje cells
of rat cerebellum were made. and enzyme activities
of succinic dehydrogenase, NADH diaphorase, lactic
dehydrogenase, acid phosphatase and thiamine pyrophosphatase
in them were observed by appropriate
enzyme cytochemical stainings.
Results obtained were as follows:
1. The blood CO-Hb concentration was 89. 1±7. 6
% in acute carbon monoxide intoxication with 0.5%
CO for 45 min. exposure and then rapidly degraded
to 20. 2±9. 4% in 60 min. past the intoxication to
leave in fresh air and reached to normal range in 4
hours past
2. Histologic sections of Nissl stainings revealed
moderate decrease of stainability in chromatophilia
of Nissl substances of cerebellar Purkinje cells and
mild eloudy swelling of them with occasional central
chromatolysis in rats of acute carbon monoxide
intoxication. These findings were aggravated as far
as 2 hours past the intoxication and thereafter arnended
gradually in 6 hours through 24 hours pas t.
3. a) Mitochondrial SDH and NADH diaphorase
activities of cerebellar Purkinje cells were moderately
decreased in acute carbon monoxide intoxication, and
their activities were gradually increased in 60 min.
through 24 hours past the intoxication and thereafter
reached to normal activities as control, to be more
or less distinct changes in the midzonal area of their
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)The Seoul Journal of MedicineThe Seoul Journal of Medicine Vol. 22 No.1 (1981)
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