급성 일산화탄소중독이 백서간장에 미치는 영향에 관한 병리조직학적 및 효소조직화학적 연구

Abstract

An experimental study was performed to observe the acute carbon monoxide poisoning in the liver of rats by means of histopathologic and enzyme histoch emical methods, attempting to explore the mechanism behind the process of the acute anoxic necrosis of liver Experimental animals(65 Sprague-Dawley rats , 210 ~280gm_) were devided into four groups by the duration of exposure and the concentration of carbon monoxide: Control (15 rats , unexposures) , 1% CO exp.(30 rats, 30 min.) , 0.7% CO exp. (12 rats,60 min.) and 0.4% CO expo (8 rats , 120 mip.). Histop hological investigation and the enzyme activities of succinic dehydrogenase (SDH) , NADH diaphorase, lactic dehydrogenase (LDH) , alkaline phosphatase (alk. P.) and acid phosphatase(acid P.) in liver were observed by hematoxylin and eosin staining and appropriate enzyme histochemical stainings. The results obtained were as follows: 1. Histologic sections of rat liver in groups of acute poisoning with carbon monoxide revealed mild to moderate congestion of central vein and sinusoidal spaces, accompanied by acidophilic degeneration of hepatocytes, pyknosis, fatty changes and focal necrosisy. Central zone was more severely affected than portal zone and the reactions were more marked in higherconcentration and shott duration groups. 2. SDH and NADH diaphorase activities in all experimental group were significantly decreased in mild to moderate degrees, especially in central and miclzonal area. 3. LDH activities in all experimental rousps generally decreased in mild degree in the central and midzone in contrast to the increase in mild to moderate degree of portal zone. 4. Changes of alk. P. activities were not noticed. Acid phosphatase activities in all experimental groups were significantly incre-ased in 60 to 120 min. expo sures. 5. Above histopathologic and histochemical findings seemed to support that acute anoxic necrosis of liver could be included by acute carbon monoxide poisoning preceded by hepatic enzymeatic changes and the most vulnerable portion of hepatic parenchyme was to be central zone of hepatic lobule.