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Enhancement of cell growth and viability of CHO cells in serum-free media by 30Kc6 gene expression

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Authors
Park, Jun Geon; Choi, Shin Sik; Park, Tai Hyun
Issue Date
2007-08
Publisher
Elsevier
Citation
Process Biochemistry, 2007;42:8-15
Keywords
30Kc6 geneSerum deprivationSerum-free mediumDG44 CHO cellSilkworm hemolymphApoptosis
Abstract
Many attempts have been made to develop a serum-free medium on account of the problems caused by serum in mammalian cell culture. However, serum deprivation inhibits cell growth and induces apoptosis. Moreover, adapting host cells to the serum-free medium is difficult and time-consuming. In a previous study, the anti-apoptotic 30K proteins were identified from silkworm hemolymph, which suggests that the 30K genes coding for the anti-apoptotic compound can be used for the anti-apoptosis engineering of mammalian cells. In this study, the 30K genes (30Kc6,30Kc19, and 30Kc123) were introduced to DG44 CHO cells, which are the mammalian cell line most commonly used by industry for the production of biopharmaceuticals, in order to make them resistant to the apoptosis induced by serum deprivation. Among the 30K genes, the 30Kc6 gene exhibited the highest apoptosis-inhibition activity. When the 30Kc6-expressing cells cultivated in the serum-containing medium were transferred directly to commercially available serum-free media, 30Kc6 expression increased the viable cell density by four-fold through inhibiting serum deprivation-induced apoptosis. (c) 2006 Elsevier Ltd. All rights reserved.
ISSN
1359-5113
Language
English
URI
http://hdl.handle.net/10371/9524
DOI
https://doi.org/10.1016/j.procbio.2006.04.010
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College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Chemical and Biological Engineering (화학생물공학부)Journal Papers (저널논문_화학생물공학부)
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