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High cell density culture of Anabaena variabilis with controlled light intensity and nutrient supply

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Authors
Yoon, Jong Hyun; Shin, Jong-Hwan; Ahn, Eun Kyung; Park, Tai Hyun
Issue Date
2008-05
Publisher
한국미생물·생명공학회 = The Korean Society for Applied Microbiology
Citation
J. Microbiol. Biotechnol. 18: 918-925
Keywords
Cyanobacteriahigh cell density culturespecific irradiation ratenutrient supplygrowth yield
Abstract
Controlling the light energy and major nutrients is important for high cell density culture of cyanobacterial cells. The growth phase of Anabaena variabilis can be divided into an exponential growth phase and a deceleration phase. In this study, the cell growth in the deceleration phase showed a linear growth pattern. Both the period of the exponential growth phase and the average cell growth rate in the deceleration phase increased by controlling the light intensity. To control the light intensity, the specific irradiation rate was maintained above 10 mu mol/s/g dry cell by increasing the incident light intensity stepwise. The final cell density increased by controlling the nutrient supply. For the control of the nutrient supply, nitrate, phosphate, and sulfate were intermittently added based on the growth yield, along with the combined control of light intensity and nutrient concentration. Under these control conditions, both final cell concentration and cell productivity increased, to 8.2 g/l and 1.9 g/l/day, respectively.
ISSN
1017-7825
Language
English
URI
http://hdl.handle.net/10371/9526
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College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Chemical and Biological Engineering (화학생물공학부)Journal Papers (저널논문_화학생물공학부)
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