Testosterone의 방사면역측정법의 정립과 한국인 아동 및 정상 성인의 혈청내 농도
Testosterone Radioimmunoassay and Serum Levels in Korean Children and Adult Males

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윤용달; 김응익; 서판길
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서울대학교 의과대학
Seoul J Med 1981;22(4):490-502
In order to improve a simple and reliable redioim'
munoassay method for the determination of testoste'
rone in human serum, various factors affecting the
assay quality were investigated: the purity of tracer;
ionic strength; pH and steroid carriers of buffer sol·
ution; and the contact time and temperature of charcoal
with antigen'antibody complex etc.
The present method was evaluatcd in terms of
accuracy, precision and the results of internal and
external quality contro l.
The concentrations (mean±SD) of testosterone in
serum from 136 healthy normal men(aged 15~50yr)
and from 116 prepubertal boys(aged 7~13yr) were
6. 85±2. 05ng/ml and O. 19±0.13, respectively. There
were no significant changes in the serum levels as
the age was increased in each group. The concentration
from 35 normal ovulatory women was 420±
70pg/ml. These values were compared with the
results of other investigators and those from several
commercial kits.
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)The Seoul Journal of MedicineThe Seoul Journal of Medicine Vol. 22 No.4 (1981)
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