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Nanoarrays of tethered lipid bilayer rafts on poly(vinyl alcohol) hydrogels

Cited 24 time in Web of Science Cited 22 time in Scopus
Authors
Lee, Bong Kuk; Lee, Hea Yeon; Kim, Pilnam; Suh, Kahp Y.; Kawai, Tomoji
Issue Date
2009
Publisher
Royal Society of Chemistry
Citation
Lab Chip, 2009, 9, 132-139
Keywords
MEMBRANE INTERACTIONSSUPPORTED MEMBRANESCELL-MEMBRANESGOLD SURFACESPROTEINSFLUIDMICROPATTERNSADSORPTIONBIOSENSORRESONANCE
Abstract
Lipid rafts are cholesterol-and sphingolipid-rich domains that function as platforms for signal transduction and other cellular processes. Tethered lipid bilayers have been proposed as a promising model to describe the structure and function of cell membranes. We report a nano(submicro) array of tethered lipid bilayer raft membranes (tLBRMs) comprising a biosensing platform. Poly(vinyl alcohol) (PVA) hydrogel was directly patterned onto a solid substrate, using ultraviolet-nanoimprint lithography (UV-NIL), as an inert barrier to prevent biofouling. The robust structures of the nanopatterned PVA hydrogel were stable for up to three weeks in phosphate-buffered saline solution despite significant swelling (100% in height) by hydration. The PVA hydrogel strongly restricted the adhesion of vesicles, resulting in an array of highly selective hydrogel nanowells. tLBRMs were not formed by direct vesicle fusion, although raft vesicles containing poly(ethylene glycol) lipopolymer were selectively immobilized on gold substrates patterned with PVA hydrogel. The deposition of tLBRM nano(submicro) arrays was accomplished by a mixed, self-assembled monolayer-assisted vesicle fusion method. The monolayer was composed of a mixture of 2-mercaptoethanol and poly(ethylene glycol) lipopolymer, which promoted vesicle rupture. These results suggest that the fabrication of inert nanostructures and the site-selective modification of solid surfaces to induce vesicle rupture may be essential in the construction of tLBRM nano(submicro) arrays using stepwise self-assembly.
ISSN
1473-0197
Language
English
URI
http://hdl.handle.net/10371/9538
DOI
https://doi.org/10.1039/b809732a
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College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Mechanical Aerospace Engineering (기계항공공학부)Journal Papers (저널논문_기계항공공학부)
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