흰쥐 시상하부내의 Vasopressin 및 Oxytocin 분비세포에 관한 면역조직화학적 연구

Abstract

To investigate the distribution of vasorpressin and oxytocin neurons, tissue sections of the hypothalamus were examined by irnmunoperoxidase technique and light microscopy using monoclonal antibodies to ox~ ytocin and arginine vasopressin Our immunocytochemical investigations reveal both vasopressin and oxytocin neurons in the supraoptic nucleus(SON) , the paraventricular nucleus(PVN) , the arcuate nucleus, the suprachiasmatic nucleus, the accessory supraoptic nucleus, the perifornical area and the periventricular area. Perikarya of these two types neurons did not show distinct morphological differences at the level of light microscopy. Among these nuclei, they were mainly distributed in the SON and PVN, and the ratio of the number of vasopressin neurons to that of oxytocin neurons was 6 : 5 in the SON, and 1 : 2 in the PVN. So the SON and PVN are indistinguishable in that both cell types are present in both nuclei, but impressively vasopressin neurons were found only in the posterior half of the PVN