생쥐에서의 수정란 이식에 관한 연구
A Study on the Embryo Transfer in Mouse

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서울대학교 의과대학
Seoul J Med 1984;25(4):455-466
Superovulation was induced in 40 C57BL/6] mice
and 40 ICR mice by the intraperitoneal injection of
5i.u. PMSG followed by 5i. u. HCG 48 hours later.
The number of oocytes ovulated, 2-cell ova, blastoc
ysts, implanted embryos. and fetuses delivered were
observed serially in superovulated group and were
compared with those of spontaneously ovulated control
group. 200 superovulated C57BL/6] blastocysts were
transferred to the uterine horns of 40 pseudopregnant
ICR mice.
The results were as follows:
1. The mean numbers of oocytes ovulated by trea
ted C57BL/G] and ICR mouse were 20.4、士4.02 and
32. 7:t9. 40 respectively, and were increased when
compared with those in controls (p<O.01) , and the proportion of abnormal oocytes which were shed was
increased in superovulated group as 10.0% and 16.0%
respectively compared with 3.0% and O. 3% in control
2. During the gestational periods, massive embryo
onic loss after superovulation was observed and 40.7%
and 44. 0% of superovulated ova were delivered to
fetuses in C57BL/6] and lCR mouse compared with
74.2% and 84. G% in control group
3. Compared to controls , the mean number of fetu'
ses was increased in superovulated group, but the
fetuses resulted in a reduction in mean fetal body
weight (p<O. OJ). The fetuses delivered following
transplantation did not represent such growth retardation
4. Implantation rate and delivery rate observed
following the surgical transfer of 200 mouse blastocysts
to the uterine horns of 40 lCR mice were 30% and
14% respectively.
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)The Seoul Journal of MedicineThe Seoul Journal of Medicine Vol. 25 No.4 (1984)
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