당뇨병 개에서 간의 포도당 조절능력에 관한 연구

Abstract

After an intravenous glucose load, a series of serum glucose, and insulin concentration ￦ere measured. in 3 diabetic dogs previously treated with alloxan, and 3 normal control dogs. Glucose disappearance rate , integrated insulin concentration, insulinogenic index, glucose uptake at tissue sites, and hepatic glucose balance were calculated and compared. Followings were the results; 1. The fasting concentration of insulin was 7.4± 231'u/ml in control group , and 3. 5±1. 31'u/ml in diabetic group (p<O. 1). Nine minutes after glucose load, the maximal insulin concentration in control group was 15.2±1. 10l'u/ml, but there was no acute response in diabetics. 2. The glucose disappearance rate was 2. 68±0. 75 %/min in control group and 2. 24±0. 51%/min, showing lower value in diabetics (p<0.15). 3. The integrated insulin concentration of the first 5 minutes was 8. 15±0. 3751lU/ml in control group, and 4. 07±0. 7161'u/ml in diabetics (p<0.05). Those of the first 10 minutes were 10.6±0. 4561'u/ml in controls, and 4. 18 土O. 3351'u/ml in diabetics (p<O.01) and those of the 60 minutes for total response were 12.1±1. 328μ띠/ml in controls, and 4. 42±0. 445μu/ml in diabetics (p<O. 025). 4. The insulinogenic index during 0~5 min and 0~10 min period for acute response and 0~60 min period for total response were as follows; 0.0147± 0.003, O. 0288±0. 009 and 0.051 ±O. 017 in controls and O. 01l5±0. 002, O. 0101±0. 002 and O. 0207±0. 013 in diabetics. (0~5 πin.; p<O. 4, 0~10 min.; p<O.l, 0~60 min.; p<0.4). 5. The glucose uptake at tissue sites was less in diabetics than in controls. 6. The hepatic glucose balance in every 10 minutes was as follows; 29.9mg/min, -45.7mg/min, -0.9 mg/min, -18.9mg/min, 45.7mg/min, 29.8mg/min and 49.0mg/min in control group , and 11. 4mg/min, 187.4mg/min, 31. 7mg/min, 16.9mg/min, 37.8mg/ min, 86.9mg/min and 78.0mg/min in diabetic dogs. From these data, it was concluded that diabetes was characterized not only by increased fasting blood glucose, lowered glucose disappearance rate, lowered insulinogenic index and glucose uptake at tissue sites but also by decreased hepatic glucose balance after intravenous glucose load.