S-Space College of Agriculture and Life Sciences (농업생명과학대학) Dept. of Food and Animal Biotechnology (식품·동물생명공학부) Journal Papers (저널논문_식품·동물생명공학부)
Detection and characterization of primordial germ cells in pheasant (Phasianus colchicus) embryos
- Kim, Jin Nam; Lee, Young Mok; Park, Tae Sub; Jung, Jin Gyoung; Cho, Byeong Wook; Lim, Jeong Mook; Han, Jae Yong
- Issue Date
- Theriogenology, vol.63 no.4, pp. 1038-1049
- The developmental similarity between the chicken and pheasant (Phasianus colchicus) allows the novel biotechnologies developed in the chicken to be applied to the production of transgenic pheasants and interspecies germline chimeras. To detect pheasant primordial germ cells (PGCs) efficiently, which is important for inducing germline transmission, the ultrastructure of PGCs and their reactivity to several antibodies (2C9, QB2, anti-SSEA-1, and QCR1) and periodic acid-Schiffs solution (PAS) were examined. To obtain PGCs, blood was taken from embryos incubated for 62–72 h or from gonads from embryos incubated for 156–216 h. The PGCs collected from both sources had the typical ultrastructure of pluripotent cells: a large nucleus with a distinct nucleolus, a high ratio of nuclear to cytoplasmic volume, and a distinct cytoplasmic membrane. In comparing the morphology of PGCs collected from different sites, more mitochondria and better-developed membrane microvilli were found in gonadal PGCs than in circulating PGCs. The nucleus of gonadal PGCs was flattened and had a large eccentrically positioned nucleolus. Of the antibodies tested, only QCR1 antibody reacted with an epitope in pheasant PGCs, and no specific signal was detected to other antibodies. The temporal change in the PGC populations in the blood and gonads of embryos was examined. In blood, the population was greater (P < 0.0001) in embryos incubated for 64 h than in embryos incubated for 62 or 66–72 h (31.4 versus 5.6–16.2 mL1). In embryonic gonads, the number of PGCs increased continuously from 156 to 216 h of incubation (193–2,718 cells/embryo), although the ratio of PGCs to total gonadal cells did not change significantly (0.50–0.61%). In conclusion, pheasant PGCs have typical germ cell morphology and possess the QCR1 epitope.
Circulating blood and the gonads of embryos incubated for 64 and 216 h, respectively, are good
sources of PGCs.
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