Induction of Apoptosis in Chicken Oniduct Cells by C2-Ceramide

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Kim, Sung Hak; Choi, Ji Young; Sihn, Choon-Ryoul; Suh, Eun Jung; Kim, Sun Young; Choi, Kang Duk; Jeon, Iksoo; Han, Jae Yong; Kim, Tae-Yoon; Kim, Sang Hoon

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The Korean Society for Molecular and Cellular Biology
Molecules and Cells, vol.19 no.2, pp. 185-190
ApoptosisCeramideChicken Oviduct Cells
The chicken oviduct is a dynamic organ that produces secretory proteins such as ovalbumin and its cells undergo cell proliferation and differentiation. There has been no study of the cellular mechanism involved in cell death in the chicken oviduct. Therefore, this study has focused on the study of apoptosis in primary oviduct cells. Because ceramide is known to activate apoptosis in tumor cells and is produced in the oviduct, we used an exogenous ceramide analog to induce cell death. The viability of ceramide-treated chicken oviduct cells decreased in a dose-dependent manner and apoptotic cells were detected by staining with annexin V. The expression of apoptosis-related genes was assessed by RT-PCR and bcl-2 mRNA was found to decrease after exposure to ceramide while Bcl-x mRNA increased 12 h post-treatment. In addition, caspase-3 was expressed strongly in the early stages of apoptosis, while caspase-1 and -9 transcripts increased at later times. We conclude that ceramide induces apoptosis in oviduct-derived primary cells via a caspase- and bcl-2-dependent pathway.
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College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Food and Animal Biotechnology (식품·동물생명공학부)Journal Papers (저널논문_식품·동물생명공학부)
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