S-Space College of Agriculture and Life Sciences (농업생명과학대학) Dept. of Food and Animal Biotechnology (식품·동물생명공학부) Journal Papers (저널논문_식품·동물생명공학부)
Identification and gene expression profiling of the Pum1 and Pum2 members of the Pumilio family in the chicken
|dc.contributor.author||Lee, Jee Young||-|
|dc.contributor.author||Lim, Jeong Mook||-|
|dc.contributor.author||Kim, Duk Kyung||-|
|dc.contributor.author||Zheng, Ying Hui||-|
|dc.contributor.author||Han, Beom Ku||-|
|dc.contributor.author||Song, Ki Duk||-|
|dc.contributor.author||Han, Jae Yong||-|
|dc.identifier.citation||Molecular Reproduction and Development, vol.75 no.1, pp. 184-190||ko_KR|
|dc.description.abstract||Members of the Pumilio (Pum) family of RNA-binding proteins act as translational repressors and are required for germ cell development and asymmetric division. We identified the chicken Pum1 and Pum2 genes and analyzed their expression patterns in various tissues. Comparative sequence analysis of the Pum1 and Pum2 proteins from the drosophila, chicken, mouse, and human revealed a high degree of evolutionary conservation in terms of the levels of homology of the peptide sequences and the structure of Pumilio homology domain (PUM-HD), Cterminal RNA-binding domain, with similar spacing between the adjacent Pum eight tandem repeats. In addition, phylogenetic patterns of pumilio family showed that Pum 1 and 2 of chicken are more closely related to those of mouse and human than other species and Pum1 is more conserved than Pum2. Using real-time RT-PCR, the expression levels of the Pum1 and Pum2 genes were found to be highest in hatched female gonads, and high-level expression of Pum2 was detected in 12-day and hatched gonads among the various chicken embryonic tissues tested. In adult tissues, the expression levels of Pum1 and Pum2 were expressed at higher levels in the testis and muscle than in any other tissue. The characteristics of the tissue-specific expression of Pum genes suggest that Pum1 and Pum2 have effects crucially in particular
stage during development of chicken gonads depending on sexual maturation.
|dc.title||Identification and gene expression profiling of the Pum1 and Pum2 members of the Pumilio family in the chicken||ko_KR|
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