Publications
Detailed Information
Development of a pheasant interspecies primordial germ cell transfer to chicken embryo: Effect of donor cell sex on chimeric semen production
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kang, S.J. | - |
dc.contributor.author | Choi, J.W. | - |
dc.contributor.author | Park, K.J. | - |
dc.contributor.author | Lee, Y.M. | - |
dc.contributor.author | Kim, T.M. | - |
dc.contributor.author | Sohn, S.H. | - |
dc.contributor.author | Lim, J.M. | - |
dc.contributor.author | Han, Jae Yong | - |
dc.date.accessioned | 2017-01-31T01:07:02Z | - |
dc.date.available | 2017-01-31T01:07:02Z | - |
dc.date.issued | 2009 | - |
dc.identifier.citation | Theriogenology, vol.72 no.4, pp. 519-527 | ko_KR |
dc.identifier.issn | 0093-691X | - |
dc.identifier.uri | https://hdl.handle.net/10371/100276 | - |
dc.description.abstract | This study was conducted to evaluate whether the sex of donor primordial germ cells (PGCs) influences production of chimeric semen from recipient hatchlings produced by interspecies transfer between pheasant (Phasianus colchicus) and chicken (Gallus gallus). Pheasant PGCs were retrieved from 7-d-old embryos and subsequently transferred into circulatory blood of 2.5-d-old (Stage 17) embryos. The sex of embryos was discerned 3 to 6 days after laying, and in preliminary study, overall rate of embryo survival after sexing was 74.6% with male-to-female ratio of 0.49 to 0.51. In Experiment 1, magnetic-activated cell sorting (MACS) using QCR1 antibody was effective for enriching the population of male and female PGCs in gonadal cells (9.2- to 12.5-fold and 10.8- to 19.5-fold increase, respectively). In Experiment 2, an increase in the number of hatchlings producing chimeric semen was detected after the homosexual transfer of male-to-male compared with that after the heterosexual transfer of female-to-male (68% to 88%). Significant increase was found in the frequency of chimeric semen production (0.96 to 1.68 times); production of pheasant progenies by artificial insemination using chimeric semen was also increased in the homosexual transfer (0 to 3 cases). In conclusion, the homosexual PGC transfer of male-to-male yielded better rate of generating pheasant progenies after test crossreproduction than that of the heterosexual transfer of female-to-male, which could improve the efficiency of interspecies germ cell transfer system. | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | Elsevier | ko_KR |
dc.subject | Chicken | ko_KR |
dc.subject | Chimerism | ko_KR |
dc.subject | Sex | ko_KR |
dc.subject | Interspecies transfer | ko_KR |
dc.subject | Pheasant | ko_KR |
dc.subject | Primordial germ cell (PGC) | ko_KR |
dc.title | Development of a pheasant interspecies primordial germ cell transfer to chicken embryo: Effect of donor cell sex on chimeric semen production | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 한재용 | - |
dc.identifier.doi | 10.1016/j.theriogenology.2009.04.007 | - |
- Appears in Collections:
- Files in This Item:
- There are no files associated with this item.
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.