Publications

Detailed Information

Expression Patterns and miRNa Regulation of DNA Methyltransferases in Chicken Primordial Germ Cells

DC Field Value Language
dc.contributor.authorRengaraj, Deivendran-
dc.contributor.authorLee, Bo Ram-
dc.contributor.authorLee, Sang In-
dc.contributor.authorSeo, Hee Won-
dc.contributor.authorHan, Jae Yong-
dc.date.accessioned2017-01-31T06:16:09Z-
dc.date.available2017-01-31T06:16:09Z-
dc.date.issued2011-
dc.identifier.citationPLoS ONE, vol.6 no.5, e19524ko_KR
dc.identifier.issn1932-6203-
dc.identifier.urihttps://hdl.handle.net/10371/100304-
dc.description.abstractDNA methylation is widespread in most species, from bacteria to mammals, and is crucial for genomic imprinting, gene expression, and embryogenesis. DNA methylation occurs via two major classes of enzymatic reactions: maintenance-type methylation catalyzed by DNA (cytosine-5-)-methyltransferase (DNMT) 1, and de novo methylation catalyzed by DNMT 3 alpha (DNMT3A) and -beta (DNMT3B). The expression pattern and regulation of DNMT genes in primordial germ cells (PGCs) and germ line cells has not been sufficiently established in birds. Therefore, we employed bioinformatics, RT-PCR, real-time PCR, and in situ hybridization analyses to examine the structural conservation and conserved expression patterns of chicken DNMT family genes. We further examined the regulation of a candidate de novo DNA methyltransferase gene, cDNMT3B by cotransfection of cDNMT3B 39UTR- and cDNMT3B 39UTR-specific miRNAs through a dual fluorescence reporter assay. All cDNMT family members were differentially detected during early embryonic development. Of interest, cDNMT3B expression was highly detected in early embryos and in PGCs. During germ line development and sexual maturation, cDNMT3B expression was reestablished in a female germ cell-specific manner. In the dual fluorescence reporter assay, cDNMT3B expression was significantly downregulated by four miRNAs: gga-miR-15c (25.82%), gga-miR-29b (30.01%), gga-miR-383 (30.0%), and gga-miR-222 (31.28%). Our data highlight the structural conservation and conserved expression patterns of chicken DNMTs. The miRNAs investigated in this study may induce downregulation of gene expression in chicken PGCs and germ cells.ko_KR
dc.language.isoenko_KR
dc.publisherPublic Library of Scienceko_KR
dc.titleExpression Patterns and miRNa Regulation of DNA Methyltransferases in Chicken Primordial Germ Cellsko_KR
dc.typeArticleko_KR
dc.contributor.AlternativeAuthor이보람-
dc.contributor.AlternativeAuthor이상인-
dc.contributor.AlternativeAuthor서희원-
dc.contributor.AlternativeAuthor한재용-
dc.identifier.doi10.1371/journal.pone.0019524-
Appears in Collections:
Files in This Item:

Altmetrics

Item View & Download Count

  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Share