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ERBB receptor feedback inhibitor 1: Identification and regulation by estrogen in chickens

Cited 1 time in Web of Science Cited 2 time in Scopus
Authors
Ahn, Suzie E.; Jeong, Wooyoung; Kim, Ji-Hye; Lim, Whasun; Kim, Jinyoung; Bazer, Fuller W.; Han, Jae Yong; Song, Gwonhwa
Issue Date
2012
Publisher
Elsevier
Citation
General and Comparative Endocrinology, vol.175 no.1, pp. 194-205
Keywords
ChickenERRFI1DESOviduct
Abstract
The ERBB receptor feedback inhibitor 1 (ERRFI1) is a scaffolding adaptor protein, that plays a pivotal role in the epidermal growth factor receptor (EGFR) cell signaling cascade as a negative regulator affecting many important physiological processes. It was recently reported that ERRFI1 is a critical regulator of the response of the endometrium to estrogen regulation of tissue homeostasis in mice. But, very little is known about ERRF11 and hormonal regulation of the ERRFI1 gene in chickens. Therefore, in the present study, ERRFI1 gene was cloned and its differential expression profile analyzed at different embryonic stages, in various adult organs, and in oviducts from estrogen-treated chickens. Chicken ERRFI1 has an open-reading frame of 2848 nucleotides that encode for a protein of 465 amino acids that has considerable homology to mammalian ERRFI1 proteins (>62% identity). Importantly, ERRFI1 mRNA is abundantly distributed in various organs from chickens. We then determined that DES (diethylstilbestrol, a synthetic nonsteroidal estrogen) induced ERRFI1 mRNA and protein predominantly in luminal and glandular epithelial cells of the oviduct. Further, we determined whether microRNAs, specifically miR-200b, miR-429 and miR-1639, influence ERRFI1 expression via its 30UTR and found that it does not directly target the 30UTR of ERRFI1 mRNA. Therefore, it is unlikely that post-transcriptional regulation influences ERRFI1 expression in the chicken oviduct. In conclusion, our results indicate that ERRFI1 is a novel estrogenstimulated gene expressed in epithelial cells of the chicken oviduct that likely plays an important role in oviduct growth and differentiation during early development of the chicken.
ISSN
0016-6480
Language
English
URI
http://hdl.handle.net/10371/100329
DOI
https://doi.org/10.1016/j.ygcen.2011.11.013
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College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Food and Animal Biotechnology (식품·동물생명공학부)Journal Papers (저널논문_식품·동물생명공학부)
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