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Gene expression profile of human peripheral b 1 lood mononuclear cells induced by 2 Staphylococcus aureus lipoteichoic acid

Cited 16 time in Web of Science Cited 16 time in Scopus
Authors
Kang, Seok-Seong; Kim, Hye Jin; Jang, Mi Seon; Moon, Sunjin; Lee, Sang In; Jeon, Jun Ho; Baik, Jung Eun; Park, Ok-Jin; Son, Young Min; Kim, Gi Rak; Joo, Donghyun; Kim, Heebal; Han, Jae Yong; Yun, Cheol-Heui; Han, Seung Hyun
Issue Date
2012
Publisher
Elsevier
Citation
International Immunopharmacology, vol.13 no.4, pp. 454-460
Keywords
Human peripheral blood mononuclear cellsLipoteichoic acidMicroarrayStaphylococcus aureusSystemic inflammatory response
Abstract
Lipoteichoic acid (LTA) is a major virulence factor of Gram-positive bacteria including Staphylococcus aureus. Despite its pivotal role in causing sepsis, the systemic immune responses to LTA in human cells are poorly understood. Here, we produced highly-pure and structurally-intact LTA from S. aureus and examined the gene expression profile of LTA-stimulated human peripheral blood mononuclear cells (PBMCs). The LTA preparation did not contain any detectable biologically-active impurities and stimulated Toll-like receptor 2. Protein expression profiling using a cytokine array kit and ELISA revealed expression of MCP-1/CCL2, IL-6, and IL-1β. We performed transcriptional profiling of PBMCs in response to S. aureus LTA using an Affymetrix genechip microarray. A total of 208 genes were significantly (fold change>1.5 and Pb0.05) altered, with 157 up-regulated and 51 down-regulated genes in response to S. aureus LTA treatment. The up-regulated genes were involved in recognition (30 genes), cellular adhesion (6 genes), signal transduction (42 genes), co-stimulation (4 genes), chemokines, cytokines and their receptors (51 genes), apoptosis (9 genes), and negative regulation (15 genes). The down-regulated genes were involved in recognition (12 genes), antigen processing and presentation (9 genes), signal transduction (27 genes), and chemotaxis (3 genes). The microarray results were validated using real-time RT-PCR with 21 up-regulated genes and 9 down-regulated genes. Our results provide a more comprehensive overview of the transcriptional changes in PBMCs in response to S. aureus LTA, and contribute to the understanding of the pathophysiological role of S. aureus LTA during the systemic inflammatory response.
ISSN
1567-5769
Language
English
URI
http://hdl.handle.net/10371/100339
DOI
https://doi.org/10.1016/j.intimp.2012.05.010
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College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Food and Animal Biotechnology (식품·동물생명공학부)Journal Papers (저널논문_식품·동물생명공학부)
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