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Cleavage Events and Sperm Dynamics in Chick Intrauterine Embryos

Cited 15 time in Web of Science Cited 23 time in Scopus
Authors
Lee, Hyung Chul; Choi, Hee Jung; Park, Tae Sub; Kim, Young Min; Rengaraj, Deivendran; Nagai, Hiroki; Sheng, Guojun; Lim, Jeong Mook; Han, Jae Yong
Issue Date
2013
Publisher
Public Library of Science
Citation
PLoS ONE, vol.8 no.11, e80631
Description
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits
unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
This study was undertaken to elucidate detailed event of early embryogenesis in chicken embryos using a noninvasive egg retrieval technique before oviposition. White Leghorn intrauterine eggs were retrieved from 95 cyclic hens aged up to 54-56 weeks and morphogenetic observation was made under both bright field and fluorescent image in a time course manner. Differing from mammals, asymmetric cleavage to yield preblastodermal cells was observed throughout early embryogenesis. The first two divisions occurred synchronously and four polarized preblastodermal cells resulted after cruciform cleavage. Then, asynchronous cleavage continued in a radial manner and overall cell size in the initial cleavage region was smaller than that in the distal area. Numerous sperms were visible, regardless of zygotic nuclei formation. Condensed sperm heads were present mainly in the perivitelline space and cytoplasm, and rarely in the yolk region, while decondensed sperm heads were only visible in the yolk. In conclusion, apparent differences in sperm dynamics and early cleavage events compared with mammalian embryos were detected in chick embryo development, which demonstrated polarized cleavage with penetrating supernumerary sperm into multiple regions.
ISSN
1932-6203
Language
English
URI
https://hdl.handle.net/10371/100372
DOI
https://doi.org/10.1371/journal.pone.0080631
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College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Food and Animal Biotechnology (식품·동물생명공학부)Journal Papers (저널논문_식품·동물생명공학부)
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