Publications

Detailed Information

The multiplex bead array approach to identifying serum biomarkers associated with breast cancer

DC Field Value Language
dc.contributor.authorKim, Byoung Kwon-
dc.contributor.authorLee, Jong Won-
dc.contributor.authorPark, Pil Je-
dc.contributor.authorShin, Yong Sung-
dc.contributor.authorLee, Won Young-
dc.contributor.authorLee, Kyung Ae-
dc.contributor.authorYe, Sena-
dc.contributor.authorHyun, Heesun-
dc.contributor.authorKang, Kyung Nam-
dc.contributor.authorYeo, Donghwa-
dc.contributor.authorKim, Youngdai-
dc.contributor.authorOhn, Sung Yup-
dc.contributor.authorNoh, Dong Young-
dc.contributor.authorKim, Chul Woo-
dc.date.accessioned2017-02-03T02:13:18Z-
dc.date.available2017-02-03T02:13:18Z-
dc.date.issued2009-04-28-
dc.identifier.citationBreast Cancer Research, 11(2):R22ko_KR
dc.identifier.urihttps://hdl.handle.net/10371/100404-
dc.descriptionThis is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0),
which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
ko_KR
dc.description.abstractAbstract

Introduction
Breast cancer is the most common type of cancer seen in women in western countries. Thus, diagnostic modalities sensitive to early-stage breast cancer are needed. Antibody-based array platforms of a data-driven type, which are expected to facilitate more rapid and sensitive detection of novel biomarkers, have emerged as a direct, rapid means for profiling cancer-specific signatures using small samples. In line with this concept, our group constructed an antibody bead array panel for 35 analytes that were selected during the discovery step. This study was aimed at testing the performance of this 35-plex array panel in profiling signatures specific for primary non-metastatic breast cancer and validating its diagnostic utility in this independent population.


Methods
Thirty-five analytes were selected from more than 50 markers through screening steps using a serum bank consisting of 4,500 samples from various types of cancer. An antibody-bead array of 35 markers was constructed using the Luminex™ bead array platform. A study population consisting of 98 breast cancer patients and 96 normal subjects was analysed using this panel. Multivariate classification algorithms were used to find discriminating biomarkers and validated with another independent population of 90 breast cancer and 79 healthy controls.


Results
Serum concentrations of epidermal growth factor, soluble CD40-ligand and proapolipoprotein A1 were increased in breast cancer patients. High-molecular-weight-kininogen, apolipoprotein A1, soluble vascular cell adhesion molecule-1, plasminogen activator inhibitor-1, vitamin-D binding protein and vitronectin were decreased in the cancer group. Multivariate classification algorithms distinguished breast cancer patients from the normal population with high accuracy (91.8% with random forest, 91.5% with support vector machine, 87.6% with linear discriminant analysis). Combinatorial markers also detected breast cancer at an early stage with greater sensitivity.


Conclusions
The current study demonstrated the usefulness of the antibody-bead array approach in finding signatures specific for primary non-metastatic breast cancer and illustrated the potential for early, high sensitivity detection of breast cancer. Further validation is required before array-based technology is used routinely for early detection of breast cancer.
ko_KR
dc.language.isoenko_KR
dc.publisherBioMed Centralko_KR
dc.titleThe multiplex bead array approach to identifying serum biomarkers associated with breast cancerko_KR
dc.typeArticleko_KR
dc.contributor.AlternativeAuthor김병권-
dc.contributor.AlternativeAuthor이종원-
dc.contributor.AlternativeAuthor박필제-
dc.contributor.AlternativeAuthor신용성-
dc.contributor.AlternativeAuthor이원영-
dc.contributor.AlternativeAuthor이경애-
dc.contributor.AlternativeAuthor예세나-
dc.contributor.AlternativeAuthor현희선-
dc.contributor.AlternativeAuthor강경남-
dc.contributor.AlternativeAuthor여동화-
dc.contributor.AlternativeAuthor김영대-
dc.contributor.AlternativeAuthor온성엽-
dc.contributor.AlternativeAuthor노동영-
dc.contributor.AlternativeAuthor김철우-
dc.identifier.doi10.1186/bcr2247-
dc.language.rfc3066en-
dc.rights.holderKim et al.; licensee BioMed Central Ltd.-
dc.date.updated2017-01-29T03:03:59Z-
Appears in Collections:
Files in This Item:

Altmetrics

Item View & Download Count

  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Share