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Separation of Mycobacterium abscessus into subspecies or genotype level by direct application of peptide nucleic acid multi-probe- real-time PCR method into sputa samples

Cited 5 time in Web of Science Cited 4 time in Scopus
Authors
Kim, Kijeong; Hong, Seok-Hyun; Kim, Byoung-Jun; Kim, Bo-Ram; Lee, So-Young; Kim, Ga-Na; Shim, Tae Sun; Kook, Yoon-Hoh; Kim, Bum-Joon
Issue Date
2015-08-11
Publisher
BioMed Central
Citation
BMC Infectious Diseases, 15(1):325
Keywords
Mycobacterium abscessusMycobacterium massiliensePeptide nucleic acid (PNA)Real time PCRhsp65Genotype
Description
This article is distributed under the terms of the Creative Commons Attribution 4.0
International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and
reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to
the Creative Commons license, and indicate if changes were made.
Abstract
Abstract

Background
Recently, we introduced a novel peptide nucleic acid (PNA) multi-probe real time PCR method targeting the hsp65 gene (hsp65 PNA RT-PCR) to distinguish Mycobacterium abscessus groups.


Methods
Here, we evaluated the usefulness of the hsp65 PNA RT-PCR for the direct identification of the M. abscessus group at the subspecies and genotype levels from sputa samples. The method was applied to total sputa DNA from 60 different patients who were identified as having mycobacterial infections via rpoB PCR restriction analysis of the same cultures.


Results
The hsp65 PNA RT-PCR method had higher sensitivity than the multi-probe real-time PCR assay targeting hsp65 (HMPRT-PCR) for the detection of M. abscessus from sputum [96.7 % (29/30 samples) vs. 70 % (21/30 samples); 100 % specificity].


Conclusions
These results suggest that the PNA-based method is feasible for the detection of M. abscessus members not only from cultures but also directly from sputa.
Language
English
URI
https://hdl.handle.net/10371/100456
DOI
https://doi.org/10.1186/s12879-015-1076-8
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)Journal Papers (저널논문_의학과)
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