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SP, CGRP changes in pyridoxine induced neuropathic dogs with nerve growth factor gene therapy

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dc.contributor.authorKang, Joo-Yeon-
dc.contributor.authorYoo, Dae Young-
dc.contributor.authorLee, Kwon-Young-
dc.contributor.authorIm, Wooseok-
dc.contributor.authorKim, Manho-
dc.contributor.authorChoi, Jung Hoon-
dc.contributor.authorYoun, Hwa-Young-
dc.contributor.authorKim, Sae Hoon-
dc.contributor.authorHwang, In Koo-
dc.contributor.authorChung, Jin-Young-
dc.date.accessioned2017-02-10T00:25:21Z-
dc.date.available2017-03-16T17:44:53Z-
dc.date.issued2016-01-05-
dc.identifier.citationBMC Neuroscience, 17(1):1ko_KR
dc.identifier.urihttps://hdl.handle.net/10371/100654-
dc.descriptionThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License
(http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium,
provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license,
and indicate if changes were made.
ko_KR
dc.description.abstractAbstract

Background
Nerve growth factor (NGF) is known not only as a major factor for neuronal plasticity but also as a pain stimulator. Although there have been several trials with NGF for its application in the regeneration or protection of the nervous system, the pain induced by NGF remains a challenge to be overcome. In this study, the pain induced by NGF gene therapy was evaluated.


Results
Vehicle or recombinant dog NGF plasmid was administered into the intrathecal space of dogs. Twenty-four hours after the vehicle or NGF plasmid inoculation, dogs were subcutaneously treated with 150 mg/kg pyridoxine every day for 7 days. For pain assessment, physical examination and electrophysiological recording were performed. Only in the vehicle-treated group, weight loss occurred, while NGF plasmid inoculation significantly improved this physical abnormalities. In the vehicle-treated group, electrophysiological recordings showed that H-reflex disappeared at 24 h after the last pyridoxine treatment. However, in the NGF plasmid inoculated group, the H-reflex were normal. In the results of immunohistochemistry, the NGF plasmid administration efficiently expressed in the dorsal root ganglia and significantly increased the pyridoxine-induced reduction of calcitonin gene-related peptide (CGRP) immunoreactive neurons, but not in substance P immunoreactive neurons, in the dorsal root ganglia.


Conclusions
Given these results, we reason that NGF gene therapy in pyridoxine induced neuropathic dogs does not induce neuropathic pain with this dosage, even with increasing the expression of CGRP.
ko_KR
dc.language.isoenko_KR
dc.publisherBioMed Centralko_KR
dc.subjectCalcitonin gene-related peptideko_KR
dc.subjectDogko_KR
dc.subjectH reflexko_KR
dc.subjectNerve growth factorko_KR
dc.subjectPyridoxineko_KR
dc.subjectNeuropathyko_KR
dc.titleSP, CGRP changes in pyridoxine induced neuropathic dogs with nerve growth factor gene therapyko_KR
dc.typeArticleko_KR
dc.contributor.AlternativeAuthor강주연-
dc.contributor.AlternativeAuthor유대영-
dc.contributor.AlternativeAuthor이권영-
dc.contributor.AlternativeAuthor임우석-
dc.contributor.AlternativeAuthor김만호-
dc.contributor.AlternativeAuthor최정훈-
dc.contributor.AlternativeAuthor윤화영-
dc.contributor.AlternativeAuthor김새훈-
dc.contributor.AlternativeAuthor황인구-
dc.contributor.AlternativeAuthor정진영-
dc.identifier.doi10.1186/s12868-015-0236-5-
dc.language.rfc3066en-
dc.rights.holderKang et al.-
dc.date.updated2017-01-06T10:22:28Z-
Appears in Collections:
College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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