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Pepper EST database: comprehensive in silico tool for analyzing the chili pepper (Capsicum annuum) transcriptome

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dc.contributor.authorKim, Hyun-Jin-
dc.contributor.authorBaek, Kwang-Hyun-
dc.contributor.authorLee, Seung-Won-
dc.contributor.authorKim, JungEun-
dc.contributor.authorLee, Bong-Woo-
dc.contributor.authorCho, Hye-Sun-
dc.contributor.authorKim, Woo Taek-
dc.contributor.authorChoi, Doil-
dc.contributor.authorHur, Cheol-Goo-
dc.date.accessioned2017-02-10T01:05:02Z-
dc.date.available2017-03-16T17:34:33Z-
dc.date.issued2008-10-09-
dc.identifier.citationBMC Plant Biology, 8(1):101ko_KR
dc.identifier.urihttps://hdl.handle.net/10371/100658-
dc.descriptionThis is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.ko_KR
dc.description.abstractBackground
There is no dedicated database available for Expressed Sequence Tags (EST) of the chili pepper (Capsicum annuum), although the interest in a chili pepper EST database is increasing internationally due to the nutritional, economic, and pharmaceutical value of the plant. Recent advances in high-throughput sequencing of the ESTs of chili pepper cv. Bukang have produced hundreds of thousands of complementary DNA (cDNA) sequences. Therefore, a chili pepper EST database was designed and constructed to enable comprehensive analysis of chili pepper gene expression in response to biotic and abiotic stresses.

Results
We built the Pepper EST database to mine the complexity of chili pepper ESTs. The database was built on 122,582 sequenced ESTs and 116,412 refined ESTs from 21 pepper EST libraries. The ESTs were clustered and assembled into virtual consensus cDNAs and the cDNAs were assigned to metabolic pathway, Gene Ontology (GO), and MIPS Functional Catalogue (FunCat). The Pepper EST database is designed to provide a workbench for (i) identifying unigenes in pepper plants, (ii) analyzing expression patterns in different developmental tissues and under conditions of stress, and (iii) comparing the ESTs with those of other members of the Solanaceae family. The Pepper EST database is freely available at http://genepool.kribb.re.kr/pepper/.

Conclusion
The Pepper EST database is expected to provide a high-quality resource, which will contribute to gaining a systemic understanding of plant diseases and facilitate genetics-based population studies. The database is also expected to contribute to analysis of gene synteny as part of the chili pepper sequencing project by mapping ESTs to the genome.
ko_KR
dc.language.isoenko_KR
dc.publisherBioMed Centralko_KR
dc.titlePepper EST database: comprehensive in silico tool for analyzing the chili pepper (Capsicum annuum) transcriptomeko_KR
dc.typeArticleko_KR
dc.contributor.AlternativeAuthor김현진-
dc.contributor.AlternativeAuthor백광현-
dc.contributor.AlternativeAuthor이승원-
dc.contributor.AlternativeAuthor김정은-
dc.contributor.AlternativeAuthor이봉우-
dc.contributor.AlternativeAuthor조혜선-
dc.contributor.AlternativeAuthor김우택-
dc.contributor.AlternativeAuthor최도일-
dc.contributor.AlternativeAuthor허철구-
dc.identifier.doi10.1186/1471-2229-8-101-
dc.language.rfc3066en-
dc.rights.holderKim et al; licensee BioMed Central Ltd.-
dc.date.updated2017-01-06T10:23:22Z-
Appears in Collections:
College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Plant Science (식물생산과학부)Journal Papers (저널논문_식물생산과학부)
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