Publications
Detailed Information
Generation of protective immunity against Orientia tsutsugamushi infection by immunization with a zinc oxide nanoparticle combined with ScaA antigen
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ha, Na-Young | - |
dc.contributor.author | Shin, Hyun Mu | - |
dc.contributor.author | Sharma, Prashant | - |
dc.contributor.author | Cho, Hyun Ah | - |
dc.contributor.author | Min, Chan-Ki | - |
dc.contributor.author | Kim, Hong-il | - |
dc.contributor.author | Yen, Nguyen Thi Hai | - |
dc.contributor.author | Kang, Jae-Seung | - |
dc.contributor.author | Kim, Ik-Sang | - |
dc.contributor.author | Choi, Myung-Sik | - |
dc.contributor.author | Kim, Young Keun | - |
dc.contributor.author | Cho, Nam-Hyuk | - |
dc.date.accessioned | 2017-02-10T02:22:49Z | - |
dc.date.available | 2017-03-16T16:51:02Z | - |
dc.date.issued | 2016-11-26 | - |
dc.identifier.citation | Journal of Nanobiotechnology, 14(1):76 | ko_KR |
dc.identifier.uri | https://hdl.handle.net/10371/100684 | - |
dc.description | This article is distributed under the terms of the Creative Commons Attribution 4.0 International License
(http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. | ko_KR |
dc.description.abstract | Abstract
Background Zinc oxide nanoparticle (ZNP) has been applied in various biomedical fields. Here, we investigated the usage of ZNP as an antigen carrier for vaccine development by combining a high affinity peptide to ZNP. Results A novel zinc oxide-binding peptide (ZBP), FPYPGGDA, with high affinity to ZNP (K a =2.26×106M−1) was isolated from a random peptide library and fused with a bacterial antigen, ScaA of Orientia tsutsugamushi, the causative agent of scrub typhus. The ZNP/ZBP-ScaA complex was efficiently phagocytosed by a dendritic cell line, DC2.4, in vitro and significantly enhanced anti-ScaA antibody responses in vivo compared to control groups. In addition, immunization with the ZNP/ZBP-ScaA complex promoted the generation of IFN-γ-secreting T cells in an antigen-dependent manner. Finally, we observed that ZNP/ZBP-ScaA immunization provided protective immunity against lethal challenge of O. tsutsugamushi, indicating that ZNP can be used as a potent adjuvant when complexed with ZBP-conjugated antigen. Conclusions ZNPs possess good adjuvant potential as a vaccine carrier when combined with an antigen having a high affinity to ZNP. When complexed with ZBP-ScaA antigen, ZNPs could induce strong antibody responses as well as protective immunity against lethal challenges of O. tsutsugamushi. Therefore, application of ZNPs combined with a specific soluble antigen could be a promising strategy as a novel vaccine carrier system. | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | BioMed Central | ko_KR |
dc.subject | Zinc oxide nanoparticle | ko_KR |
dc.subject | ZnO binding peptide | ko_KR |
dc.subject | Scrub typhus | ko_KR |
dc.subject | Vaccine | ko_KR |
dc.title | Generation of protective immunity against Orientia tsutsugamushi infection by immunization with a zinc oxide nanoparticle combined with ScaA antigen | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 하나영 | - |
dc.contributor.AlternativeAuthor | 신현무 | - |
dc.contributor.AlternativeAuthor | 조현아 | - |
dc.contributor.AlternativeAuthor | 민찬기 | - |
dc.contributor.AlternativeAuthor | 김홍일 | - |
dc.contributor.AlternativeAuthor | 강재승 | - |
dc.contributor.AlternativeAuthor | 김익상 | - |
dc.contributor.AlternativeAuthor | 최명식 | - |
dc.contributor.AlternativeAuthor | 김영근 | - |
dc.contributor.AlternativeAuthor | 조남혁 | - |
dc.identifier.doi | 10.1186/s12951-016-0229-2 | - |
dc.language.rfc3066 | en | - |
dc.rights.holder | The Author(s) | - |
dc.date.updated | 2017-01-06T10:27:11Z | - |
- Appears in Collections:
- Files in This Item:
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.