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Complete genome of Vibrio parahaemolyticus FORC014 isolated from the toothfish

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dc.contributor.authorAhn, Sojin-
dc.contributor.authorChung, Han Young-
dc.contributor.authorLim, Sooyeon-
dc.contributor.authorKim, Kwondo-
dc.contributor.authorKim, Suyeon-
dc.contributor.authorNa, Eun Jung-
dc.contributor.authorCaetano-Anolles, Kelsey-
dc.contributor.authorLee, Ju-Hoon-
dc.contributor.authorRyu, Sangryeol-
dc.contributor.authorChoi, Sang Ho-
dc.contributor.authorKim, Heebal-
dc.date.accessioned2017-02-10T04:11:30Z-
dc.date.available2017-02-10T04:11:30Z-
dc.date.issued2016-11-17-
dc.identifier.citationGut Pathogens, 8(1):59ko_KR
dc.identifier.urihttps://hdl.handle.net/10371/100688-
dc.descriptionThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.ko_KR
dc.description.abstractBackground
Foodborne illness can occur due to various pathogenic bacteria such as Staphylococcus aureus, Escherichia coli and Vibrio parahaemolyticus, and can cause severe gastroenteritis symptoms. In this study, we completed the genome sequence of a foodborne pathogen V. parahaemolyticus FORC_014, which was isolated from suspected contaminated toothfish from South Korea. Additionally, we extended our knowledge of genomic characteristics of the FORC_014 strain through comparative analysis using the complete sequences of other V. parahaemolyticus strains whose complete genomes have previously been reported.

Results
The complete genome sequence of V. parahaemolyticus FORC_014 was generated using the PacBio RS platform with single molecule, real-time (SMRT) sequencing. The FORC_014 strain consists of two circular chromosomes (3,241,330 bp for chromosome 1 and 1,997,247 bp for chromosome 2), one plasmid (51,383 bp), and one putative phage sequence (96,896 bp). The genome contains a total of 4274 putative protein coding sequences, 126 tRNA genes and 34 rRNA genes. Furthermore, we found 33 type III secretion system 1 (T3SS1) related proteins and 15 type III secretion system 2 (T3SS2) related proteins on chromosome 1. This is the first reported result of Type III secretion system 2 located on chromosome 1 of V. parahaemolyticus without thermostable direct hemolysin (tdh) and thermostable direct hemolysin-related hemolysin (trh).

Conclusions
Through investigation of the complete genome sequence of V. parahaemolyticus FORC_014, which differs from previously reported strains, we revealed two type III secretion systems (T3SS1, T3SS2) located on chromosome 1 which do not include tdh and trh genes. We also identified several virulence factors carried by our strain, including iron uptake system, hemolysin and secretion system. This result suggests that the FORC_014 strain may be one pathogen responsible for foodborne illness outbreak. Our results provide significant genomic clues which will assist in future understanding of virulence at the genomic level and help distinguish between clinical and non-clinical isolates.
ko_KR
dc.language.isoenko_KR
dc.publisherBioMed Centralko_KR
dc.subjectVibrio parahaemolyticusko_KR
dc.subjectType III secretion system-2ko_KR
dc.subjectWhole genome sequencingko_KR
dc.subjectComparative genomicsko_KR
dc.titleComplete genome of Vibrio parahaemolyticus FORC014 isolated from the toothfishko_KR
dc.typeArticleko_KR
dc.contributor.AlternativeAuthor안소진-
dc.contributor.AlternativeAuthor정한영-
dc.contributor.AlternativeAuthor임수연-
dc.contributor.AlternativeAuthor김권도-
dc.contributor.AlternativeAuthor김수연-
dc.contributor.AlternativeAuthor나은정-
dc.contributor.AlternativeAuthor이주훈-
dc.contributor.AlternativeAuthor류상렬-
dc.contributor.AlternativeAuthor최상호-
dc.contributor.AlternativeAuthor김희발-
dc.identifier.doi10.1186/s13099-016-0134-0-
dc.language.rfc3066en-
dc.rights.holderThe Author(s)-
dc.date.updated2017-01-06T10:27:54Z-
Appears in Collections:
College of Agriculture and Life Sciences (농업생명과학대학)Program in Agricultural Biotechnology (협동과정-농업생물공학전공)Journal Papers (저널논문_협동과정-농업생물공학전공)
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