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Suicide cancer gene therapy using pore-forming toxin, streptolysin O
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yang, W. S. | - |
dc.contributor.author | Park, S. O. | - |
dc.contributor.author | Yoon, A. R. | - |
dc.contributor.author | Yoo, J. Y. | - |
dc.contributor.author | Kim, M. K. | - |
dc.contributor.author | Yun, C. O. | - |
dc.contributor.author | Kim, C. W. | - |
dc.date.accessioned | 2009-10-06T03:07:36Z | - |
dc.date.available | 2009-10-06T03:07:36Z | - |
dc.date.issued | 2006-07-05 | - |
dc.identifier.citation | Mol Cancer Ther 2006;5:1610-9. | en |
dc.identifier.issn | 1535-7163 | - |
dc.identifier.uri | https://hdl.handle.net/10371/10186 | - |
dc.description.abstract | We cloned the streptolysin O gene from the Streptococcus pyogenes genome and tested the possibility of using it as an anticancer reagent. Transient transfection of the streptolysin O gene efficiently killed 293T cells after 12 hours of transfection as determined by lactate dehydrogenase release and propidium iodide uptake. No caspase activity was observed and necrosis was prominent during streptolysin O-induced cell death. Biochemical analysis of streptolysin O protein revealed that the deletion of only 5 amino acids from the COOH-terminal region of streptolysin O, which is essential for cholesterol binding activity, abolished its cell-killing activity, whereas the NH2-terminal region was more resilient, i.e., up to 115 amino acids could be deleted without changing its cell-killing activity. We generated a streptolysin O-expressing adenovirus and injected it into human cervical cancer cell-derived tumors grown in a nude mouse model. Twenty-one days postinjection, the average size of tumors in the streptolysin O adenovirus-injected group was 29.3% of that of the control PBS-treated group. Our results show that the genes of pore-forming toxins, like streptolysin O protein, have the potential to establish a novel class of suicide gene therapeutic reagents. | en |
dc.description.sponsorship | Korea Science & Engineering Foundation through the
Tumor Immunity Medical Research Center at Seoul National University College of Medicine. | en |
dc.language.iso | en | en |
dc.publisher | American Association for Cancer Research | en |
dc.subject | Adenoviridae/genetics | en |
dc.subject | Animals | en |
dc.subject | Apoptosis | en |
dc.subject | Bacterial Proteins/genetics | en |
dc.subject | Female | en |
dc.subject | Gene Expression | en |
dc.subject | Genes, Transgenic, Suicide/*physiology | en |
dc.subject | Genetic Vectors | en |
dc.subject | Green Fluorescent Proteins/metabolism | en |
dc.subject | Humans | en |
dc.subject | Male | en |
dc.subject | Mice | en |
dc.subject | Mice, Nude | en |
dc.subject | Streptococcus pyogenes/*genetics | en |
dc.subject | Streptolysins/*genetics | en |
dc.subject | Transfection | en |
dc.subject | Tumor Cells, Cultured | en |
dc.subject | Uterine Cervical Neoplasms/metabolism/*therapy | en |
dc.subject | Xenograft Model Antitumor Assays | en |
dc.subject | Gene Therapy | - |
dc.title | Suicide cancer gene therapy using pore-forming toxin, streptolysin O | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 양완석 | - |
dc.contributor.AlternativeAuthor | 박수오 | - |
dc.contributor.AlternativeAuthor | 윤아름 | - |
dc.contributor.AlternativeAuthor | 유지영 | - |
dc.contributor.AlternativeAuthor | 김민경 | - |
dc.contributor.AlternativeAuthor | 윤재옥 | - |
dc.contributor.AlternativeAuthor | 김철우 | - |
dc.identifier.doi | 10.1158/1535-7163.MCT-05-0515 | - |
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