Publications
Detailed Information
Single-cell mRNA sequencing identifies subclonal heterogeneity in anti-cancer drug responses of lung adenocarcinoma cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Kyu-Tae | - |
dc.contributor.author | Lee, Hye Won | - |
dc.contributor.author | Lee, Hae-Ock | - |
dc.contributor.author | Kim, Sang Cheol | - |
dc.contributor.author | Seo, Yun Jee | - |
dc.contributor.author | Chung, Woosung | - |
dc.contributor.author | Eum, Hye Hyeon | - |
dc.contributor.author | Nam, Do-Hyun | - |
dc.contributor.author | Kim, Junhyong | - |
dc.contributor.author | Joo, Kyeung Min | - |
dc.contributor.author | Park, Woong-Yang | - |
dc.date.accessioned | 2017-03-20T07:25:31Z | - |
dc.date.available | 2017-03-20T16:48:02Z | - |
dc.date.issued | 2015-06-19 | - |
dc.identifier.citation | Genome Biology, 16(1):127 | ko_KR |
dc.identifier.uri | https://hdl.handle.net/10371/109878 | - |
dc.description.abstract | Background
Intra-tumoral genetic and functional heterogeneity correlates with cancer clinical prognoses. However, the mechanisms by which intra-tumoral heterogeneity impacts therapeutic outcome remain poorly understood. RNA sequencing (RNA-seq) of single tumor cells can provide comprehensive information about gene expression and single-nucleotide variations in individual tumor cells, which may allow for the translation of heterogeneous tumor cell functional responses into customized anti-cancer treatments. Results We isolated 34 patient-derived xenograft (PDX) tumor cells from a lung adenocarcinoma patient tumor xenograft. Individual tumor cells were subjected to single cell RNA-seq for gene expression profiling and expressed mutation profiling. Fifty tumor-specific single-nucleotide variations, including KRAS G12D, were observed to be heterogeneous in individual PDX cells. Semi-supervised clustering, based on KRAS G12D mutant expression and a risk score representing expression of 69 lung adenocarcinoma-prognostic genes, classified PDX cells into four groups. PDX cells that survived in vitro anti-cancer drug treatment displayed transcriptome signatures consistent with the group characterized by KRAS G12D and low risk score. Conclusions Single-cell RNA-seq on viable PDX cells identified a candidate tumor cell subgroup associated with anti-cancer drug resistance. Thus, single-cell RNA-seq is a powerful approach for identifying unique tumor cell-specific gene expression profiles which could facilitate the development of optimized clinical anti-cancer strategies. | ko_KR |
dc.language.iso | en | ko_KR |
dc.publisher | BioMed Central | ko_KR |
dc.title | Single-cell mRNA sequencing identifies subclonal heterogeneity in anti-cancer drug responses of lung adenocarcinoma cells | ko_KR |
dc.type | Article | ko_KR |
dc.contributor.AlternativeAuthor | 김규태 | - |
dc.contributor.AlternativeAuthor | 이혜원 | - |
dc.contributor.AlternativeAuthor | 이해옥 | - |
dc.contributor.AlternativeAuthor | 김상철 | - |
dc.contributor.AlternativeAuthor | 서윤지 | - |
dc.contributor.AlternativeAuthor | 정우성 | - |
dc.contributor.AlternativeAuthor | 엄혜현 | - |
dc.contributor.AlternativeAuthor | 남도현 | - |
dc.contributor.AlternativeAuthor | 김준형 | - |
dc.contributor.AlternativeAuthor | 주경민 | - |
dc.contributor.AlternativeAuthor | 박웅양 | - |
dc.language.rfc3066 | en | - |
dc.rights.holder | Kim et al. | - |
dc.date.updated | 2017-01-06T10:44:11Z | - |
- Appears in Collections:
- Files in This Item:
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.