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Respiratory syncytial virus infection induces matrix metalloproteinase-9 expression in epithelial cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yeo, S.-J. | - |
dc.contributor.author | Yun, Y.-J. | - |
dc.contributor.author | Lyu, M.-A. | - |
dc.contributor.author | Woo, S.-Y. | - |
dc.contributor.author | Woo, E.-R. | - |
dc.contributor.author | Kim, S.-J. | - |
dc.contributor.author | Lee, H.-J. | - |
dc.contributor.author | Park, H.-K. | - |
dc.contributor.author | Kook, Y.-H. | - |
dc.date.accessioned | 2009-11-04T05:28:07Z | - |
dc.date.available | 2009-11-04T05:28:07Z | - |
dc.date.issued | 2002-03-14 | - |
dc.identifier.citation | Arch Virol 147: 229-242 | en |
dc.identifier.issn | 0304-8608 (Print) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=11890521 | - |
dc.identifier.uri | https://hdl.handle.net/10371/11113 | - |
dc.description.abstract | Increased gelatinolytic activity was observed in respiratory syncytial virus (RSV)-infected HEp-2 cells by using zymography. The anti-matrix metalloproteinase-9 (MMP-9) antibody specifically reduced the gelatinolytic activity suggesting that the increased gelatinolytic activity was due to the MMP-9. It was also supported by the results from immunofluorescent staining, treatment of MMP inhibitors, and RSV infection of the cell clones that were transfected with plasmids to express more MMP-9 and tissue type inhibitor of metalloproteinase-1 (TIMP-1). The gelatinolytic activity of extracellular MMP-9 in RSV-infected HEp-2 cells increased 1.5 +/- 0.2 fold compared with the control (p < 0.01). Cell surface MMP-9 expression was also clearly detected by immunofluorescent staining. Treatment with 1,10-phenanthroline (0.05 mM), ethylenediamine-tetraacetate (EDTA) (1.5 mM), and penta-O-galloyl-beta-D-glucose (PGG) (3.3 microM) inhibited RSV multiplication as well as syncytia formation. Furthermore, the average syncytia size increased when the cells expressing more MMP-9 were infected by RSV. In contrast, syncytia formation was inhibited in the cells manipulated to express TIMP-1. Thus, this study concludes that although RSV infection induces MMP-9, which can enhance the syncytia formation leading to RSV multiplication and spread it can be inhibited by MMP inhibitors. | en |
dc.language.iso | en | - |
dc.publisher | Springer Verlag | en |
dc.subject | Epithelial Cells/*metabolism/virology | en |
dc.subject | Giant Cells/physiology | en |
dc.subject | Matrix Metalloproteinase 9/antagonists & inhibitors/genetics/*metabolism | en |
dc.subject | Respiratory Syncytial Virus Infections/*virology | en |
dc.subject | Respiratory Syncytial Viruses/*pathogenicity/physiology | en |
dc.subject | Tissue Inhibitor of Metalloproteinase-1/genetics/metabolism | en |
dc.subject | Transfection | en |
dc.subject | Tumor Cells, Cultured | en |
dc.subject | Virus Replication | en |
dc.title | Respiratory syncytial virus infection induces matrix metalloproteinase-9 expression in epithelial cells | en |
dc.type | Article | en |
dc.identifier.doi | 10.1007/s705-002-8316-1 | - |
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