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Identification of autoantibodies associated with systemic lupus erythematosus

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Authors
Lim, Yoon; Lee, Dae-Yeon; Lee, Seongeun; Park, Sae-Young; Kim, Jongwan; Cho, Bomsoo; Lee, Hosoon; Kim, Hae-Yeong; Lee, Eunbong; Song, Yeong Wook; Jeoung, Doo-Il
Issue Date
2002-06-27
Publisher
Elsevier
Citation
Biochem. Biophys. Res. Commun. 295 (2002) 119-124
Keywords
cDNA expression libraryPARPSEREXSystemic lupus erythematosus
Abstract
Systemic lupus erythematosus (SLE) is an autoimmune disease characterized by the presence of antinuclear antibodies. We performed serological analysis of cDNA expression library (SEREX) to identify autoantibodies associated with SLE. The screening of three different cDNA expression libraries with pooled sera of patients with SLE yielded 11 independent clones that reacted with pooled sera of patients with SLE. In this screening, autoantibodies to poly(ADP-ribose) polymerase (PARP), U1snRNP, and galectin-3 were prevalent in the sera of patients with SLE (26/68, 25/68, 12/63, respectively). The frequency of autoantibody to PARP was significantly higher in SLE than that of healthy donors (0/76) (38.2% vs 0%, p<0.00001). The autoantibody to PARP was infrequently detected in the serum of patients with RA (1/50). However, autoantibody to PARP was not found in the sera of patients with other rheumatic diseases including Sjogren's syndrome (0/19), systemic sclerosis (0/18), and polymyositis/myositis (0/37). The frequency of autoantibody to human galectin-3 (12/63) was significantly higher in SLE than that of healthy donors (0/56) (19% vs 0%, p=0.0006). Autoantibody to galectin-3 was not found in the sera of patients with rheumatoid arthritis (0/50), Sjogren's syndrome (0/18), and systemic sclerosis (0/19). Interestingly, autoantibody to galectin-3 was also prevalent in the sera of patients with polymyositis/dermatomyositis (16/37, 43.2%). Further functional characterization of these autoantibodies would be necessary to determine their value as diagnostic markers or to define clinical subsets of patients with SLE. Statistical analysis revealed that the presence of autoantibody to PARP was inversely related with pleurisy, and the presence of autoantibody to galectin-3 related with renal disease.
ISSN
0006-291X (Print)
Language
English
URI
http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WBK-464P997-S&_user=404106&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000019238&_version=1&_urlVersion=0&_userid=404106&md5=965e0dcade86afe060e3f540a4f59edb

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12083777

http://hdl.handle.net/10371/11140
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College of Medicine/School of Medicine (의과대학/대학원)Internal Medicine (내과학전공)Journal Papers (저널논문_내과학전공)
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