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Effect of Rheum Palmatum on in vitro and in vivo ruminal methane production and bacterial community analysis based on 16S rRNA sequence

Cited 6 time in Web of Science Cited 6 time in Scopus
Authors
Kim, Kyoung Hoon; Arokiyaraj, Selvaraj; Lee, Jinwook; Oh, Young Kyoon; Kim, Eun Bae; Chung, Hoyoung; Choi, Seong Ho; Kim, Do Hyoung; Lee, Yoonseok
Issue Date
2016-02-09
Publisher
CSIRO PUBLISHING
Citation
Animal Production Science, Vol. 56 No. 2-3, pp. 402-408
Keywords
bacterial communitybeef cattlerhubarbruminal methane
Abstract
The objective of this study was to evaluate the anti-methanogenic effect of rhubarb (Rheum spp.) on in vitro and in vivo. Rhubarb root powder was tested at different concentrations (0, 0.33, 0.67, and 1.33 g/L) in vitro, and all incubations were carried out in triplicate two runs on separate days. Concentrations of 0.67 and 1.33 g/L rhubarb significantly (P < 0.05) reduced methane production and the acetate propionate ratio compared with those of the control, without adverse effects on total volatile fatty acids and total gas production. In the second in vivo trial, four Hanwoo (Korean native) steers (live body weight 556 ± 46 kg) with a ruminal cannula were housed individually in metabolic stalls and fed a basal diet twice daily in equal amounts at 0900 and 2100 hours. The before rhubarb treatment (before treatment) duration was 24 days for all steers; 14 days were used for diet adaptation and 10 days were used for gas samples collected 1, 2, and 3 hours after the morning feeding on days 3, 5, 7, and 9. We used three syringe needles passed through the ruminal cannula stopper at different time points. Thereafter, mesh bag containing 90g sliced rhubarb root was placed in the rumen of each steer for 14 days 4, 7, 10, 12, and 13. The results showed a significant (P < 0.05) decrease in methane concentration from the rhubarb-treated steers. Furthermore, 16s RNA sequencing after treatment showed an increase in the numbers of Prevotella and decreases in Ruminococcus and Methanobrevibacter. In conclusion, rhubarb had an anti-methanogenic effect in vitro and in vivo, and the increase in the number of Prevotella shifted ruminal fermentation toward propionate production.
ISSN
1836-0939
Language
English
URI
https://hdl.handle.net/10371/116825
DOI
https://doi.org/10.1071/AN15585
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Graduate School of International Agricultural Technology (국제농업기술대학원)Dept. of International Agricultural Technology (국제농업기술학과)Journal Papers (저널논문_국제농업기술학과)
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