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Immune adjuvant effect of a Toxoplasma gondii profilin-like protein in autologous whole-tumor-cell vaccination in mice
27687589

Cited 10 time in Web of Science Cited 13 time in Scopus
Authors
Pyo, Kyoung-Ho; Lee, You-Won; Lim, Sun Min; Shin, Eun-Hee
Issue Date
2016-09-28
Publisher
IMPACT JOURNALS LLC
Citation
ONCOTARGET Vol.7 No.45, pp. 74107-74119
Keywords
Toxoplasma gondiiToll-Like Receptorprofilinantitumorvaccine
Abstract
Profilin-like protein in Toxoplasma gondii (TgPLP) is a Toll-like receptor (TLR) agonist. In this study, we investigated whether TgPLP has an adjuvant effect on immune function in autologous whole-tumor-cell vaccine (AWV) treatment. Mice vaccinated with AWV together with recombinant TgPLP protein had smaller CT26 tumors and increased survival. TgPLP treatment strongly increased the production of IL-12 through MyD88 signaling and several chemokines, including CCL5, CCL12, and XCL1, in bone marrow-derived macrophages (BMMs). In addition, TgPLP increased the phagocytosis of tumor cells by BMMs and promoted immune cell mobility on a tumor-matrigel scaffold. TgPLP triggered immune responses as demonstrated by increased expression of antigen presenting cell markers (MHC class I and II, B7.1, and B7.2) in BMMs and increased IL-12 and IFN-gamma expression in mice. Mice vaccinated with AWV and TgPLP had more immune cells (CD4(+) and CD8(+) T cells, natural killer cells, and macrophages) in the spleen and higher total IgG and IgG2a concentrations in the blood than mice vaccinated with AWV alone. These findings suggest that TgPLP is a TLR-based vaccine adjuvant that enhances antitumor immune responses during vaccination with AWV.
ISSN
1949-2553
Language
English
URI
http://hdl.handle.net/10371/116986
DOI
https://doi.org/10.18632/oncotarget.12316
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)Journal Papers (저널논문_의학과)
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