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Isolation and Characterization of Pepper Genes Interacting with the CMV-P1 Helicase Domain

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dc.contributor.authorChoi, Yoomien
dc.contributor.authorKang, Min-Young-
dc.contributor.authorLee, Joung-Ho-
dc.contributor.authorKang, Won-Hee-
dc.contributor.authorHwang, JeeNa-
dc.contributor.authorKwon, Jin-Kyung-
dc.contributor.authorKang, Byoung-Cheorl-
dc.creator강병철-
dc.date.accessioned2017-04-25T07:47:05Z-
dc.date.available2017-12-08T17:04:18Z-
dc.date.issued2016-01-
dc.identifier.citationPLoS ONE, Vol. 11 No.1, pp.1-14-
dc.identifier.issn1932-6203-
dc.identifier.urihttps://hdl.handle.net/10371/117533-
dc.description.abstractCucumber mosaic virus (CMV) is a destructive pathogen affecting Capsicum annuum (pepper) production. The pepper Cmr1 gene confers resistance to most CMV strains, but is overcome by CMV-P1 in a process dependent on the CMV-P1 RNA1 helicase domain (P1 helicase). Here, to identify host factors involved in CMV-P1 infection in pepper, a yeast two-hybrid library derived from a C. annuum 'Bukang' cDNA library was screened, producing a total of 76 potential clones interacting with the P1 helicase. Beta-galactosidase filter lift assay, PCR screening, and sequencing analysis narrowed the candidates to 10 genes putatively involved in virus infection. The candidate host genes were silenced in Nicotiana benthamiana plants that were then inoculated with CMV-P1 tagged with the green fluorescent protein (GFP). Plants silenced for seven of the genes showed development comparable to N. benthamiana wild type, whereas plants silenced for the other three genes showed developmental defects including stunting and severe distortion. Silencing formate dehydrogenase and calreticulin-3 precursor led to reduced virus accumulation. Formate dehydrogenase- silenced plants showed local infection in inoculated leaves, but not in upper (systemic) leaves. In the calreticulin-3 precursor-silenced plants, infection was not observed in either the inoculated or the upper leaves. Our results demonstrate that formate dehydrogenase and calreticulin-3 precursor are required for CMV-P1 infection.en
dc.description.sponsorshipThis study was supported by a grant from the Next-Generation BioGreen 21 Program (The Agricultural Genome Center No. PJ011204), Rural Development Administration, and from the Vegetable Breeding Research Center through the R&D Convergence Center Support Program, Ministry of Agriculture, Food and Rural Affairs (MAFRA), Republic of Korea.-
dc.language.isoen-
dc.publisherPUBLIC LIBRARY SCIENCEen
dc.subjectIsolation and Characterization of Pepper Genes Interacting with the CMV-P1 Helicase Domainen
dc.subject복합학en
dc.titleIsolation and Characterization of Pepper Genes Interacting with the CMV-P1 Helicase Domainen
dc.typeArticle-
dc.contributor.AlternativeAuthor최유미-
dc.contributor.AlternativeAuthor강민영-
dc.contributor.AlternativeAuthor이정호-
dc.contributor.AlternativeAuthor강원희-
dc.contributor.AlternativeAuthor황지나-
dc.contributor.AlternativeAuthor권진경-
dc.contributor.AlternativeAuthor강병철-
dc.identifier.doi10.1371/journal.pone.0146320-
dc.citation.journaltitlePLoS ONE-
dc.description.srndOAIID:RECH_ACHV_DSTSH_NO:T201625252-
dc.description.srndRECH_ACHV_FG:RR00200001-
dc.description.srndADJUST_YN:-
dc.description.srndEMP_ID:A076900-
dc.description.srndCITE_RATE:3.057-
dc.description.srndFILENAME:PLOS ONE(2016) Isolation and characterization of pepper genes interacting with CMVP1 helicase domain.PDF-
dc.description.srndDEPT_NM:식물생산과학부-
dc.description.srndEMAIL:bk54@snu.ac.kr-
dc.description.srndSCOPUS_YN:Y-
dc.description.srndFILEURL:https://srnd.snu.ac.kr/eXrepEIR/fws/file/e808d5fe-49fa-4e89-9b04-a8faab286170/link-
dc.description.srndCONFIRM:Y-
dc.identifier.rimsid10605-
dc.identifier.srndT201625252-
dc.type.rimsART-
Appears in Collections:
College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Plant Science (식물생산과학부)Journal Papers (저널논문_식물생산과학부)
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