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Fine mapping of Restorer-of-fertility in pepper (Capsicum annuum L.) identified a candidate gene encoding a pentatricopeptide repeat (PPR)-containing protein

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dc.contributor.authorJo, Yeong Deuken
dc.contributor.authorHa, Yeaseong-
dc.contributor.authorLee, Joung‑Ho-
dc.contributor.authorPark, Minkyu-
dc.contributor.authorBergsma, Alex C.-
dc.contributor.authorChoi, Hong‑Il-
dc.contributor.authorGoritschnig, Sandra-
dc.contributor.authorKloosterman, Bjorn-
dc.contributor.authorvan Dijk, Peter J.-
dc.contributor.authorChoi, Doil-
dc.contributor.authorKang, Byoung‑Cheorl-
dc.date.accessioned2017-04-25T07:47:07Z-
dc.date.available2017-07-19T10:06:24Z-
dc.date.issued2016-07-28-
dc.identifier.citationTHEORETICAL AND APPLIED GENETICS Vol.129 No.10, pp. 2003-2017-
dc.identifier.issn0040-5752-
dc.identifier.urihttps://hdl.handle.net/10371/117535-
dc.identifier.uri10.1007/s00122-016-2755-6-
dc.description.abstractKey message
Using fine mapping techniques, the genomic region co-segregating with Restorer-of-fertility (Rf) in pepper was delimited to a region of 821 kb in length. A PPR gene in this region, CaPPR6, was identified as a strong candidate for Rf based on expression pattern and characteristics of encoding sequence.
Abstract
Cytoplasmic-genic male sterility (CGMS) has been used for the efficient production of hybrid seeds in peppers (Capsicum annuum L.). Although the mitochondrial candidate genes that might be responsible for cytoplasmic male sterility (CMS) have been identified, the nuclear Restorer-of-fertility (Rf) gene has not been isolated. To identify the genomic region co-segregating with Rf in pepper, we performed fine mapping using an Rf-segregating population consisting of 1068 F2 individuals, based on BSA-AFLP and a comparative mapping approach. Through six cycles of chromosome walking, the co-segregating region harboring the Rf locus was delimited to be within 821 kb of sequence. Prediction of expressed genes in this region based on transcription analysis revealed four candidate genes. Among these, CaPPR6 encodes a pentatricopeptide repeat (PPR) protein with PPR motifs that are repeated 14 times. Characterization of the CaPPR6 protein sequence, based on alignment with other homologs, showed that CaPPR6 is a typical Rf-like (RFL) gene reported to have undergone diversifying selection during evolution. A marker developed from a sequence near CaPPR6 showed a higher prediction rate of the Rf phenotype than those of previously developed markers when applied to a panel of breeding lines of diverse origin. These results suggest that CaPPR6 is a strong candidate for the Rf gene in pepper.
en
dc.language.isoenen
dc.publisherSPRINGERen
dc.subjectFine mapping of Restorer-of-fertility in pepper (Capsicum annuum L.) identified a candidate gene encoding a pentatricopeptide repeat (PPR)-containing proteinen
dc.subject자연과학en
dc.titleFine mapping of Restorer-of-fertility in pepper (Capsicum annuum L.) identified a candidate gene encoding a pentatricopeptide repeat (PPR)-containing proteinen
dc.typeArticle-
dc.contributor.AlternativeAuthor조영득-
dc.contributor.AlternativeAuthor하예성-
dc.contributor.AlternativeAuthor이정호-
dc.contributor.AlternativeAuthor박민규-
dc.contributor.AlternativeAuthor최홍일-
dc.contributor.AlternativeAuthor최도일-
dc.contributor.AlternativeAuthor강병철-
dc.description.srndOAIID:RECH_ACHV_DSTSH_NO:T201625308-
dc.description.srndRECH_ACHV_FG:RR00200001-
dc.description.srndADJUST_YN:-
dc.description.srndEMP_ID:A076900-
dc.description.srndCITE_RATE:3.9-
dc.description.srndFILENAME:TAG(2016) Fine mapping of Rf in pepper.pdf-
dc.description.srndDEPT_NM:식물생산과학부-
dc.description.srndEMAIL:bk54@snu.ac.kr-
dc.description.srndSCOPUS_YN:Y-
dc.description.srndFILEURL:https://srnd.snu.ac.kr/eXrepEIR/fws/file/8c5822f8-5600-4a60-a386-0529e2f49934/link-
dc.description.srndCONFIRM:Y-
dc.identifier.rimsid10163-
dc.identifier.srndT201625308-
dc.type.rimsART-
Appears in Collections:
College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Plant Science (식물생산과학부)Journal Papers (저널논문_식물생산과학부)
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