S-Space College of Agriculture and Life Sciences (농업생명과학대학) Program in Agricultural Biotechnology (협동과정-농업생물공학전공) Journal Papers (저널논문_협동과정-농업생물공학전공)
Comparative genomic analysis reveals genetic features related to the virulence of Bacillus cereus FORC_013
- Koo, Hyun-Jin; Ahn, Sojin; Chung, Han Young; Kim, Suyeon; Kim, Kwondo; Ryu, Sangryeol; Lee, Ju-Hoon; Choi, Sang Ho; Kim, Heebal
- Issue Date
- BioMed Central
- Gut Pathogens, 9(1):29
Bacillus cereus is well known as a gastrointestinal pathogen that causes food-borne illness. In the present study, we sequenced the complete genome of B. cereus FORC_013 isolated from fried eel in South Korea. To extend our understanding of the genomic characteristics of FORC_013, we conducted a comparative analysis with the published genomes of other B. cereus strains.
We fully assembled the single circular chromosome (5,418,913 bp) and one plasmid (259,749 bp); 5511 open reading frames (ORFs) and 283 ORFs were predicted for the chromosome and plasmid, respectively. Moreover, we detected that the enterotoxin (NHE, HBL, CytK) induces food-borne illness with diarrheal symptom, and that the pleiotropic regulator, along with other virulence factors, plays a role in surviving and biofilm formation. Through comparative analysis using the complete genome sequence of B. cereus FORC_013, we identified both positively selected genes related to virulence regulation and 224 strain-specific genes of FORC_013.
Through genome analysis of B. cereus FORC_013, we identified multiple virulence factors that may contribute to pathogenicity. These results will provide insight into further studies regarding B. cereus pathogenesis mechanism at the genomic level.