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G-CSF down-regulation of CXCR4 expression identified as a mechanism for mobilization of myeloid cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Hyun Kyung | - |
dc.contributor.author | De La Luz Sierra, Maria | - |
dc.contributor.author | Williams, Cassin Kimmel | - |
dc.contributor.author | Gulino, A Virginia | - |
dc.contributor.author | Tosato, Giovanna | - |
dc.date.accessioned | 2009-11-10T14:41:53Z | - |
dc.date.available | 2009-11-10T14:41:53Z | - |
dc.date.issued | 2006-08-01 | - |
dc.identifier.citation | Blood. 2006;108:812-820 | en |
dc.identifier.issn | 0006-4971 (Print) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16537807 | - |
dc.identifier.uri | https://hdl.handle.net/10371/11870 | - |
dc.description.abstract | CXCR4 receptor expression is required for the retention of granulocyte precursors and mature neutrophils within the bone marrow, and disruption of the SDF-1/CXCR4 axis in the bone marrow results in the mobilization of myeloid lineage cells to the peripheral circulation. We report that G-CSF down-regulates CXCR4 expression in bone marrow-derived murine and human myeloid lineage cells. When exposed to G-CSF, murine Gr1(+) bone marrow myeloid cells display a time-dependent reduction of cell-surface CXCR4 and respond poorly to SDF-1 in attachment and migration assays. Bone marrow-derived cells of nonmyeloid lineage display no change in surface CXCR4 expression upon exposure to G-CSF. Compared with controls, mice treated with G-CSF for mobilization of hematopoietic progenitor cells display reduced levels of CXCR4 selectively in bone marrow Gr1(+) myeloid cells. Since bone marrow myeloid cells express G-CSF receptors and G-CSF rapidly reduces CXCR4 expression in purified Gr1(+) cells populations, these results provide evidence that G-CSF acts directly on myeloid lineage cells to reduce CXCR4 expression. By down-regulating CXCR4 expression in bone marrow myeloid cells and attenuating their responsiveness to SDF-1, G-CSF promotes their mobilization from the bone marrow to the peripheral blood. | en |
dc.language.iso | en | en |
dc.publisher | American Society of Hematology | en |
dc.subject | Bone Marrow Cells | en |
dc.subject | Chemokine CXCL12 | en |
dc.subject | Chemokines, CXC/physiology | en |
dc.subject | Down-Regulation/*drug effects/genetics | en |
dc.subject | Granulocyte Colony-Stimulating Factor/administration & | en |
dc.subject | dosage/*pharmacology | en |
dc.subject | Hematopoietic Stem Cell Mobilization/*methods | en |
dc.subject | Mice, Inbred C57BL | en |
dc.subject | Myeloid Cells/drug effects/metabolism | en |
dc.subject | Receptors, CXCR4/*drug effects/genetics | en |
dc.title | G-CSF down-regulation of CXCR4 expression identified as a mechanism for mobilization of myeloid cells | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 김현경 | - |
dc.identifier.doi | 10.1182/blood-2005-10-4162 | - |
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