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Green tea extract inhibits paraquat-induced pulmonary fibrosis by suppression of oxidative stress and endothelin-l expression
DC Field | Value | Language |
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dc.contributor.author | Kim, Hak-Ryul | - |
dc.contributor.author | Park, Byung-Kyu | - |
dc.contributor.author | Oh, Yeon-Mok | - |
dc.contributor.author | Lee, Yun-Song | - |
dc.contributor.author | Lee, Dong-Soon | - |
dc.contributor.author | Kim, Hyun-Kuk | - |
dc.contributor.author | Kim, Joo-Young | - |
dc.contributor.author | Shim, Tae-Sun | - |
dc.contributor.author | Lee, Sang-Do | - |
dc.date.accessioned | 2009-11-10T14:44:17Z | - |
dc.date.available | 2009-11-10T14:44:17Z | - |
dc.date.issued | 2006 | - |
dc.identifier.citation | Lung 184:287-295 | en |
dc.identifier.issn | 0341-2040 (Print) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17235729 | - |
dc.identifier.uri | https://hdl.handle.net/10371/11871 | - |
dc.description.abstract | Paraquat-induced pulmonary fibrosis involves two factors, direct injury by oxygen free radicals and indirect injury by inflammatory cells and fibroblasts. Endothelin-1 (ET-1) has been shown to act as a mediator of pulmonary fibrosis, and its formation increases during oxidative stress. We investigated whether green tea extract (GTE), which has antioxidant properties, inhibits paraquat-induced pulmonary fibrosis and whether ET-1 is involved in this process. Paraquat (0.3 mg/kg) was instilled into the right lungs of rats, following which the rats were either not further treated (Group P, n = 7), or they were administered 1% GTE mixed with feed (Group PG; n = 7) or the ET(A) receptor antagonist ZD2574 (10 mg/kg through gavage; Group PZ; n = 7) for two weeks. As control, we used rats instilled with saline (Group N; n = 6). Two weeks after paraquat instillation, we assayed the degree of pulmonary fibrosis by light microscopic morphometry and hydroxyproline content; lipid peroxidation as a marker of oxidative stresses by measurement of malondialdehyde (MDA); ET-1 by immunohistochemistry; and prepro-ET-1 mRNA expression by reverse transcription-polymerase chain reaction. Compared with Group N, significant pulmonary fibrosis was observed in Group P, accompanied by increases in MDA, ET-1, and prepro-ET-1 mRNA expression. Compared with Group P, Group PG showed significant decreases in pulmonary fibrosis, along with decreases in MDA, ET-1, and prepro-ET-1 mRNA expression. We also observed significant decreases in pulmonary fibrosis in Group PZ compared with Group P. These findings suggest that GTE inhibits paraquat-induced pulmonary fibrosis by suppression of oxidative stress and ET-1 expression. | en |
dc.language.iso | en | en |
dc.publisher | Springer Verlag | en |
dc.subject | Camellia sinensis | en |
dc.subject | Disease Models, Animal | en |
dc.subject | Endothelin-1/biosynthesis/*genetics | en |
dc.subject | Gene Expression/*drug effects | en |
dc.subject | Herbicides/toxicity | en |
dc.subject | Immunohistochemistry | en |
dc.subject | Malondialdehyde/metabolism | en |
dc.subject | Oxidative Stress/*drug effects | en |
dc.subject | Paraquat/toxicity | en |
dc.subject | Plant Extracts/*therapeutic use | en |
dc.subject | Pulmonary Fibrosis/chemically induced/*drug therapy/metabolism | en |
dc.subject | RNA/*genetics | en |
dc.subject | Rats, Sprague-Dawley | en |
dc.subject | Reverse Transcriptase Polymerase Chain Reaction | en |
dc.subject | Treatment Outcome | en |
dc.title | Green tea extract inhibits paraquat-induced pulmonary fibrosis by suppression of oxidative stress and endothelin-l expression | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 김학렬 | - |
dc.contributor.AlternativeAuthor | 박병규 | - |
dc.contributor.AlternativeAuthor | 오연목 | - |
dc.contributor.AlternativeAuthor | 이윤송 | - |
dc.contributor.AlternativeAuthor | 이동순 | - |
dc.contributor.AlternativeAuthor | 김현국 | - |
dc.contributor.AlternativeAuthor | 김주영 | - |
dc.contributor.AlternativeAuthor | 심태선 | - |
dc.contributor.AlternativeAuthor | 이상도 | - |
dc.identifier.doi | 10.1007/s00408-005-2592-x | - |
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