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Green tea extract inhibits paraquat-induced pulmonary fibrosis by suppression of oxidative stress and endothelin-l expression
Cited 40 time in
Web of Science
Cited 40 time in Scopus
- Authors
- Issue Date
- 2006
- Publisher
- Springer Verlag
- Citation
- Lung 184:287-295
- Keywords
- Camellia sinensis ; Disease Models, Animal ; Endothelin-1/biosynthesis/*genetics ; Gene Expression/*drug effects ; Herbicides/toxicity ; Immunohistochemistry ; Malondialdehyde/metabolism ; Oxidative Stress/*drug effects ; Paraquat/toxicity ; Plant Extracts/*therapeutic use ; Pulmonary Fibrosis/chemically induced/*drug therapy/metabolism ; RNA/*genetics ; Rats, Sprague-Dawley ; Reverse Transcriptase Polymerase Chain Reaction ; Treatment Outcome
- Abstract
- Paraquat-induced pulmonary fibrosis involves two factors, direct injury by oxygen free radicals and indirect injury by inflammatory cells and fibroblasts. Endothelin-1 (ET-1) has been shown to act as a mediator of pulmonary fibrosis, and its formation increases during oxidative stress. We investigated whether green tea extract (GTE), which has antioxidant properties, inhibits paraquat-induced pulmonary fibrosis and whether ET-1 is involved in this process. Paraquat (0.3 mg/kg) was instilled into the right lungs of rats, following which the rats were either not further treated (Group P, n = 7), or they were administered 1% GTE mixed with feed (Group PG; n = 7) or the ET(A) receptor antagonist ZD2574 (10 mg/kg through gavage; Group PZ; n = 7) for two weeks. As control, we used rats instilled with saline (Group N; n = 6). Two weeks after paraquat instillation, we assayed the degree of pulmonary fibrosis by light microscopic morphometry and hydroxyproline content; lipid peroxidation as a marker of oxidative stresses by measurement of malondialdehyde (MDA); ET-1 by immunohistochemistry; and prepro-ET-1 mRNA expression by reverse transcription-polymerase chain reaction. Compared with Group N, significant pulmonary fibrosis was observed in Group P, accompanied by increases in MDA, ET-1, and prepro-ET-1 mRNA expression. Compared with Group P, Group PG showed significant decreases in pulmonary fibrosis, along with decreases in MDA, ET-1, and prepro-ET-1 mRNA expression. We also observed significant decreases in pulmonary fibrosis in Group PZ compared with Group P. These findings suggest that GTE inhibits paraquat-induced pulmonary fibrosis by suppression of oxidative stress and ET-1 expression.
- ISSN
- 0341-2040 (Print)
- Language
- English
- URI
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17235729
https://hdl.handle.net/10371/11871
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