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Rapid drug susceptibility test of M. tuberculosis using single cell tracking method in agarose matrix
아가로즈 내에 단일 세포 추적 방법을 이용한 결핵균의 신속한 약제 감수성 검사 기법

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dc.contributor.advisor권성훈-
dc.contributor.author최정일-
dc.date.accessioned2017-07-13T07:07:42Z-
dc.date.available2017-07-13T07:07:42Z-
dc.date.issued2015-02-
dc.identifier.other000000025316-
dc.identifier.urihttps://hdl.handle.net/10371/119062-
dc.description학위논문 (박사)-- 서울대학교 대학원 : 전기·컴퓨터공학부, 2015. 2. 권성훈.-
dc.description.abstractIn this thesis, I describe a principle and system for rapid drug susceptibility test (DST) of M. tuberculosis (MTB). In the test system, single cell of MTB is immobilized in the agarose matrix and culture media with TB drugs are diffused into the agarose. The response of single cell is observed by microscopy and the images were transformed by image processing program for quantification. In case of common pathogen, antibiotic susceptibility test comparable with standard method was performed in 3 ~ 4 hours. Single cell morphological analysis is developed to handle the heterogeneous responses of bacteria in the various antimicrobial condition and the system derived an AST result satisfying the FDA standard for new AST systems. In the final, DST system for MTB was developed which can determine the DST of MDT in one week by single cell tracking method. Single cell tracking method in agarose matrix is validated as a solution for tackle the global health problem of tuberculosis and antibiotic resistance.-
dc.description.tableofcontents1.Introduction 1
2.Background 3
2.1Need of rapid drug susceptibility test 3
2.2The conventional DST methods 5
2.3Single cell tracking method for rapid DST 8
3.Rapid antibiotic susceptibility testing by tracking single cell growth 10
3.1AST process in the MAC system 11
3.1.1AST process in the MAC system 11
3.1.2Interface formation between the agarose-bacteria mixture and the liquid medium 14
3.1.3Fabrication of the PDMS MAC chip 17
3.1.4Bacteria, chemicals and incubation conditions 18
3.2Rapid AST in the MAC system 18
3.2.1Diffusion of antibiotics into agarose 18
3.2.2Bacterial fixation and tracking bacterial single cell growth by using the MAC system 20
3.2.3Image processing 22
3.2.4AST with the PDMS MAC system 24
3.3Summary 30
4.Single-Cell Morphological Analysis 31
4.1AST process in the plastic MAC system 33
4.1.1Microfluidic agarose channel (MAC) integration with a 96-well format 33
4.1.2Diffusion characteristics in agarose within the MAC chip 39
4.2Single-cell morphological analysis (SCMA) for rapid and accurate AST 47
4.2.1Development of SCMA 47
4.2.2Automated susceptibility determination based on SCMA 68
4.2.3SCMA for determination of antimicrobial susceptibility in clinical samples 79
4.2.4SCMA reduces AST error rates compared with the bacterial area measuring (BAM) method 83
4.2.5Discussion 86
4.3Summary 91
5.Rapid DST of M.tuberculosis by single cell tracking method 92
5.1DST process in the disk agarose culture (DAC) system 93
5.1.1Design on a chip and its fabrication process 93
5.1.2DST process 96
5.2Rapid DST of M.tuberculosis using the DAC system 98
5.2.1A 3D culture formation chip for the DST of MTB 98
5.2.2Diffusion characteristics in the DAC chip 99
5.2.3Rapid DST using single cell tracking in the DAC system 102
5.2.4Discussion 107
5.3Summary 109
6.Conculusions and Future works 110
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dc.formatapplication/pdf-
dc.format.extent4561377 bytes-
dc.format.mediumapplication/pdf-
dc.language.isoen-
dc.publisher서울대학교 대학원-
dc.subjectTuberculosis-
dc.subjectAntibiotic resistant-
dc.subjectSingle cell tracking-
dc.subjectAgarose-
dc.subjectDrug susceptibility test-
dc.subject.ddc621-
dc.titleRapid drug susceptibility test of M. tuberculosis using single cell tracking method in agarose matrix-
dc.title.alternative아가로즈 내에 단일 세포 추적 방법을 이용한 결핵균의 신속한 약제 감수성 검사 기법-
dc.typeThesis-
dc.contributor.AlternativeAuthorChoi Jungil-
dc.description.degreeDoctor-
dc.citation.pagesxvii, 119-
dc.contributor.affiliation공과대학 전기·컴퓨터공학부-
dc.date.awarded2015-02-
Appears in Collections:
College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Electrical and Computer Engineering (전기·정보공학부)Theses (Ph.D. / Sc.D._전기·정보공학부)
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