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Characterization of genes related to heat shock response in Fusarium graminearum : Fusarium graminearum의 열충격반응 관련 유전자 기능연구
DC Field | Value | Language |
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dc.contributor.advisor | Yin-Won Lee | - |
dc.contributor.author | Bui Duc Cuong | - |
dc.date.accessioned | 2017-07-13T08:24:48Z | - |
dc.date.available | 2017-07-13T08:24:48Z | - |
dc.date.issued | 2016-08 | - |
dc.identifier.other | 000000136710 | - |
dc.identifier.uri | https://hdl.handle.net/10371/119523 | - |
dc.description | 학위논문 (박사)-- 서울대학교 대학원 : 농생명공학부 식물미생물전공, 2016. 8. 이인원. | - |
dc.description.abstract | The Fusarium head blight primarily caused by ascomycete Fusarium graminearum leads to severe yield losses of various cereal crops and contamination of grains with mycotoxins that are harmful to humans and livestock. The heat shock response involves the induction of a defined set of heat shock proteins which promote the folding of client proteins or target aggregated proteins for degradation, therefore affecting the diverse cellular processes in eukaryotes. In this study, genome-wide transcriptome analysis of cellular adaptation to thermal stress was performed in F. graminearum. We found that profound alterations in gene expression were required for heat shock response in F. graminearum. Heat shock protein 90 (FgHsp90) played a central role in heat shock response and FgHsp90 was exclusively localized to nuclei in response to heat stress. Moreover, the comprehensive functional characterization of FgHsp90 provides clear genetic evidences supporting its crucial roles in vegetative growth, reproduction, and virulence of F. graminearum. Next, the FgNot3 subunit of the Ccr4-Not complex, evolutionarily conserved from yeast to human, was investigated in this fungus. FgNot3 had numerous functions in fungal morphogenesis, sporulation, and virulence. We found that FgNot3 functions as a negative regulator of the production of secondary metabolites, including trichothecenes and zearalenone. Numerous defects of Fgnot2 and Fgnot4 deletion mutants, other subunit mutants of the Ccr4-Not complex, demonstrated that the Not module of the Ccr4-Not complex is conserved, and each subunit not only primarily functions within the context of a complex, but also might have distinct roles outside of the complex in F. graminearum. This is the first study to functionally characterize the Hsp90 and the Ccr4-Not complex in plant pathogenic fungi and provides novel insights into signal transduction pathways in fungal development. | - |
dc.description.tableofcontents | CHAPTER 1. Heat shock protein 90 is required for sexual and asexual development, virulence, and heat shock response in Fusarium graminearum 1
ABSTRACT 2 INTRODUCTION 3 MATERIALS AND METHODS 7 I. Fungal strains and media 7 II. Nucleic acid manipulation, primers, and PCR conditions 7 III. Genetic modifications 12 IV. Conidial production 13 V. Sexual development and virulence tests 13 VI. Microscopic observation 14 VII. qRT-PCR 15 VIII. RNA-seq and bioinformatic analysis 15 RESULTS 17 I. Heat shock causes a profound modification of gene expression in F. graminearum 17 II. Transcription of FgHSP90 is induced in response to heat stress and during sexual and asexual development 23 III. Molecular characterization of FgHsp90 26 IV. FgHSP90 is an essential gene in F. graminearum 30 V. FgHSP90 is required for conidiation in F. graminearum 34 VI. FgHSP90 is involved in virulence and sexual development 36 VII. The subcellular localization pattern of FgHsp90 reveals its multiple functions during the conidiation stage 41 DISCUSSION 46 LITERATURE CITED 50 CHAPTER 2. The FgNot3 subunit of the Ccr4-Not complex regulates vegetative growth, sporulation, and virulence in Fusarium graminearum 58 ABSTRACT 59 INTRODUCTION 60 MATERIALS AND METHODS 64 I. Fungal strains and media 64 II. Nucleic acid manipulation, primers, and PCR conditions 66 III. Genetic manipulations and fungal transformations 66 IV. Conidial production and morphology 67 V. Germination assay 68 VI. Outcrosses and virulence test 68 VII. Quantification of mycotoxins and fungal ergosterol 69 VIII. Quantitative real-time (qRT)-PCR 70 IX. Yeast strains and complementation assay 71 RESULTS 73 I. Molecular characterization of the FgNOT3 gene 73 II. Effects of FgNOT3 deletion on vegetative growth, conidiogenesis, and germination 78 III. FgNOT3 is important for sexual development and virulence 86 IV. FgNOT3 is required for normal growth under high-temperature conditions 89 V. FgNot3 functions together with other Not subunits of the Ccr4-Not complex 89 VI. FgNOT2, FgNOT3, and FgNOT4 are all involved in secondary metabolite production 100 DISCUSSION 102 LITERATURE CITED 116 ABSTRACT IN KOREAN 125 | - |
dc.format | application/pdf | - |
dc.format.extent | 3254009 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | Fusarium graminearum | - |
dc.subject | Fusarium head blight | - |
dc.subject | heat shock protein 90 | - |
dc.subject | Ccr4-Not complex | - |
dc.subject | fungal development | - |
dc.subject | conidiation | - |
dc.subject | sporulation | - |
dc.subject | virulence | - |
dc.subject.ddc | 630 | - |
dc.title | Characterization of genes related to heat shock response in Fusarium graminearum | - |
dc.title.alternative | Fusarium graminearum의 열충격반응 관련 유전자 기능연구 | - |
dc.type | Thesis | - |
dc.contributor.AlternativeAuthor | 부이둑쿠옹 | - |
dc.description.degree | Doctor | - |
dc.citation.pages | 137 | - |
dc.contributor.affiliation | 농업생명과학대학 농생명공학부 | - |
dc.date.awarded | 2016-08 | - |
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