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Evaluation of Type 1 Porcine Reproductive and Respiratory Syndrome Virus Vaccines based on Clinical, Virological, Immunological and Pathological Analyses
유럽형 돼지 생식기 호흡기 증후군 바이러스 백신의 임상학적, 바이러스학적, 면역학적, 병리학적 분석을 통한 평가

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dc.contributor.advisor채찬희-
dc.contributor.author김태연-
dc.date.accessioned2017-07-13T16:44:50Z-
dc.date.available2017-07-13T16:44:50Z-
dc.date.issued2016-02-
dc.identifier.other000000132405-
dc.identifier.urihttps://hdl.handle.net/10371/120231-
dc.description학위논문 (박사)-- 서울대학교 대학원 : 수의과대학 수의학과 수의병인생물학및예방수의학전공, 2016. 2. 채찬희.-
dc.description.abstractThe Porcine Reproductive and Respiratory Syndrome Virus was first recognized in the early 1900s as a cause of reproductive losses in sow and severe respiratory disorders in growing pigs. PRRSV is recognized by 2 genotypes: Type 1 (European genotype) and Type 2 (North American genotype). Although these two PRRSV genotypes produce the same disease symptoms, they are significantly different antigens. Until late 2000s, Type 2 PRRSV was the main genotype in Korea. After the first detection of Type 1 PRRSV in 2005, the proportion of Type 1 PRRSV has continuously increased. Until 2013, Type 2 PRRSV vaccines were the only vaccines available in Korea. However, the modified live virus (MLV) vaccines for Type 1 PRRSV became available in 2014. Therefore, in order to evaluate the efficacies of commercial vaccines, it is reasonable to measure the cell-mediated immunity, humoral immunity, pathological and clinical responses against these two types of PRRSV.
A study was performed to compare the efficacies of two commercial Type 1 PRRSV MLV vaccines against heterologous Type 1 and Type 2 PRRSV challenge in growing pigs and boars. For the study of growing pigs, a total of 112 pigs were randomly divided into 7 groups
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dc.description.abstract4 vaccinated and challenged groups, 2 non-vaccinated and challenged groups, and a negative control group. According to this study, commercial Type 1 PRRSV MLV vaccines could reduce the level of viremia against Type 1 PRRSV, but it could not reduce the level of viremia against Type 2 PRRSV. Low level of IL-10 and high level of Type 1 PRRSV-specific IFN-γ-SC were both detected within Type 1 PRRSV challenged groups, whereas high level of IL-10 and low number of Type 2 PRRSV-specific IFN-γ-SC were detected in vaccinated pigs within Type 2 PRRSV challenged groups. Type 1 PRRSV vaccines effectively reduced the lung lesions and Type 1 PRRSV nucleic acids in Type 1 PRRSV challenged groups. However, both the lung lesions and Type 2 PRRSV nucleic acids did not reduce in Type 2 PRRSV challenged groups. Accordingly, the Type 1 PRRSV commercial vaccines can provide partial protections against Type 1 PRRSV challenge but cannot provide effective protection against heterologous Type 2 PRRSV challenge.
For the study of boars, a total of 35 purebred Landrace boars were randomly divided into 7 groups
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dc.description.abstract4 vaccinated and challenged groups, 2 non-vaccinated and challenged groups, and a negative control group. In this study, the clinical symptoms were reduced by Type 1 PRRSV MLV vaccines after challenging with Type 1 and Type 2 PRRSV. Seminal shedding of PRRSV was independent of viremia. The reduction of Type 1 PRRSV seminal shedding coincided with the appearance of Type 1 PRRSV-specific IFN-γ-SC in Type 1 PRRSV-challenged groups. The frequencies of Type 1 PRRSV-specific IFN-γ-SC induced by Type 1 PRRSV vaccine were relatively high compared to Type 2 PRRSV-specific IFN-γ-SC induced by the same vaccine. The PRRSV MLV vaccine can provide a more effective protection against the same genotype than a different genotype in terms of seminal shedding of PRRSV in boars.-
dc.description.tableofcontentsGENERAL INTRODUCTION 1

LITERATURE REVIEW 4
1. Etiology 4
2. Clinical sign and Pathogenesis 6
3. Economic loss 8
4. Control 9
5. Vaccine and Immune responses 10
6. References 13

PART I. Comparison of Two Commercial Type 1 Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Modified Live Vaccines against Heterologous Type 1 and Type 2 PRRSV Challenge in Growing Pigs 23
Abstract 24
Introduction 25
Materials and methods 27
Results 35
Discussion 47
References 51

PART II. Two Commercial Type 1 Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) Modified Live Vaccines Reduce Seminal Shedding of Type 1 PRRSV but not Type 2 PRRSV in Infected Boars 57
Abstract 58
Introduction 59
Materials and methods 61
Results 69
Discussion 79
References 81

GENERAL CONCLUSION 88

ABSTRACT IN KOREAN 90
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dc.formatapplication/pdf-
dc.format.extent1957547 bytes-
dc.format.mediumapplication/pdf-
dc.language.isoen-
dc.publisher서울대학교 대학원-
dc.subjectBoar-
dc.subjectPiglet-
dc.subjectCross-protection-
dc.subjectPorcine Reproductive and Respiratory Syndrome Virus-
dc.subjectSemen-
dc.subjectVaccine-
dc.subjectVaccine efficacy-
dc.subject.ddc636-
dc.titleEvaluation of Type 1 Porcine Reproductive and Respiratory Syndrome Virus Vaccines based on Clinical, Virological, Immunological and Pathological Analyses-
dc.title.alternative유럽형 돼지 생식기 호흡기 증후군 바이러스 백신의 임상학적, 바이러스학적, 면역학적, 병리학적 분석을 통한 평가-
dc.typeThesis-
dc.contributor.AlternativeAuthorKim Taeyeon-
dc.description.degreeDoctor-
dc.citation.pages102-
dc.contributor.affiliation수의과대학 수의학과-
dc.date.awarded2016-02-
Appears in Collections:
College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Theses (Ph.D. / Sc.D._수의학과)
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