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GBS-based map construction and QTL identification for major agronomic traits in mungbean

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dc.contributor.advisor이석하-
dc.contributor.author황원주-
dc.date.accessioned2017-07-13T17:38:43Z-
dc.date.available2017-07-13T17:38:43Z-
dc.date.issued2015-02-
dc.identifier.other000000025773-
dc.identifier.urihttps://hdl.handle.net/10371/120998-
dc.description학위논문 (박사)-- 서울대학교 대학원 : 식물생산과학부, 2015. 2. 이석하.-
dc.description.abstractMungbean is an important crop grown in South, East and Southeast Asia. As mungbean contains a high protein content, along with other nutritional befinits such as carboyhoydrates, lipids, minerals, and vitamins, it is important to investigate genomics regions controlling specific agronomic traits for the improvement in breeding programs, where most of the major traits are quantitatively inherited. The general objective is to identify quantitative trait loci (QTLs) of mungbean associated with days to first flowering (DFF), days to 50% flowering (DF), 100-seed weight (100-SW), lodging resistance (Ldge), and cercospora leaf spot resistance (CLS). Flowering is one of the most crucial trait to be studied in mungbean because of its direct correlation with crop yield. It has previously been reported that a higher number of pod yield produces after the two to three weeks of first flowering and its consequences of a high yielding mungbean. Therefore, it is of great interest to investigate the genetic loci underlying flowering time and its associated traits, allowing breeders to develop novel varieties with efficient altered flowering times. In this study, a total of 190 F6 recombinant inbred lines (RILs) developed from the cross of VC 1973A (Sunhwa nokdu) and VC 2984 (Gyeonggi Jaerae #5) were used to identify quantitative trait loci (QTLs) for DFF, DF, 100-SW, Ldge, CLS, plant height (PH), and number of branches (NB).
A genetic map was constructed using 798 single nucleotide polymorphism (SNP) markers developed by a genotyping-by-sequencing (GBS) method from a segregating a RIL population. The generated markers were distributed over all 11 chromosomes (Chrs), covering 757.39 centiMorgans (cM) of mungbean genome with the average distance between adjacent markers of 0.95 cM. Using composite interval mapping (CIM), we identified six QTLs were identified for DFF and DF, four for 100-SW, two for PH, two for NB, one for Ldge, and two for CLS. Among them, one QTL located on Chr4 was identified to be associated with three agronomics traits with significant LOD scores, of which they were DFF, DF and PH. This genomic region then was deployed in the soybean genome for comparative analysis to identify synteny relationships between mungbean and soybean, whether this region co-localized with the soybean flowering QTL regions.
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dc.description.tableofcontentsCONTENTS

GENERAL ABSTRACT i
LIST OF FIGURES vi
LIST OF TABLES vii
LIST OF ABBREVIATIONS viii
GENERAL INTRODUCTION 9
LITERATURAL REVIEWS 13
Genotyping by Sequencing 13
QTL Analysis 15
Genetic linkage map of mungbean 18
REFERENCES 20
CHAPTER I 24
GBS-based map construction and QTL identification for major agronomic traits in mungbean 24
ABSTRACT 25
INTRODUCTION 27
MATERIAL AND METHODS 30
Plant population and Agronomic traits 30
Single nucleotide polymorphism (SNP) markers development 31
Statistical analysis 32
RESULTS 33
Variation of agronomic traits 33
Correlations among major agronomic traits 36
QTL analysis of agronomic traits 37
DISCUSSION 41
CHAPTER II 44
QTL for flowering traits in mungbean was associated with soybean flowering genes 44
ABSTRACT 45
INTRODUCTION 47
MATERIALS AND METHODS 52
Plant population 52
Phenotypic evaluation 52
Genetic map and QTL analysis 53
Comparative analysis of the QTL regions for DFF and DF 54
Analysis of whole genome duplication (WGD) 55
RESULTS 57
Phenotype data analysis 57
QTL analysis of DFF and DF 60
Comparative analysis of the QTL regions for DFF and DF 64
Discussion 68
REFERENCES 73
국문 초록 83
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dc.formatapplication/pdf-
dc.format.extent1140727 bytes-
dc.format.mediumapplication/pdf-
dc.language.isoen-
dc.publisher서울대학교 대학원-
dc.subjectMungbean QTL-
dc.subject.ddc633-
dc.titleGBS-based map construction and QTL identification for major agronomic traits in mungbean-
dc.typeThesis-
dc.contributor.AlternativeAuthorHWANG WON JOO-
dc.description.degreeDoctor-
dc.citation.pagesviii, 85-
dc.contributor.affiliation농업생명과학대학 식물생산과학부-
dc.date.awarded2015-02-
Appears in Collections:
College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Plant Science (식물생산과학부)Theses (Ph.D. / Sc.D._식물생산과학부)
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