Physically Crosslinked and Chemically Photocrosslinked Silk Hydrogel Manipulated via Molecular Weight Control : 분자량조절기법을 이용하여 제조한 물리적 가교 및 화학적 광가교 실크 하이드로젤

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농업생명과학대학 바이오시스템·소재학부
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서울대학교 대학원
Silk fibroinMethacrylateHydrogelAlkaline hydrolysisPhysical crosslinkingChemical crosslinkingPhoto-crosslinking
학위논문 (박사)-- 서울대학교 대학원 : 바이오시스템·소재학부, 2017. 2. 박영환.
In this study, alkaline hydrolysis was utilized using heat-alkaline treatment (HAT) method to manipulate the silk hydrogel properties. By regulating the hydrolysis time (10-180 min), a broad molecular weight range of silk fibroin (SF) could be obtained (77.2-258.6 kDa). The change of molecular weight of SF also greatly affected the physical properties (i.e., swelling ratio, shear modulus, transparency) of SF hydrogel. As a result of structural analysis, the molecular weight of SF played a crucial role in the construction of microstructure of SF hydrogel. These findings indicate that physically crosslinked SF hydrogels of variable physical properties can be fabricated based on molecular weight control. However, this manipulation could not improve the mechanical property (i.e., brittleness) of typical SF hydrogel in addition to a long gelation time. Chemical crosslinking of SF can overcome these problems by making strong covalent bond in a network within predictable gelation time. Therefore, new strategy was developed for making chemically photo-crosslinked SF hydrogel without using of fresh SF aqueous solution. By lowering the molecular weight of SF, the stability of SF aqueous solution could be enhanced and consequently, this allowed direct chemical modification of SF. Subsequently, photo-crosslinkable silk fibroin methacrylate (SFMA) was synthesized using the hydrolyzed SF and chemically photo-crosslinked SF hydrogel (SFMA hydrogel) could be fabricated with a rapid gel formation. The structural characteristics, physical properties, and performance of chemically crosslinked SF hydrogel were intensively examined on the effect of immobilized MA amount on SF and molecular weight of SF. It is expected that SFMA hydrogel has a high potential use in biomedical applications due to its excellent gel properties and performance (e.g., transparency, resiliency, and injectability).
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College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Biosystems and Biomaterials Science and Engineering (바이오시스템·소재학부)Theses (Ph.D. / Sc.D._바이오시스템·소재학부)
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