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TNF-alpha-mediated apoptosis in chondrocytes sensitized by MG132 or actinomycin D
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Hyun A. | - |
dc.contributor.author | Song, Yeong W. | - |
dc.date.accessioned | 2009-11-13T04:51:48Z | - |
dc.date.available | 2009-11-13T04:51:48Z | - |
dc.date.issued | 2002 | - |
dc.identifier.citation | Biochem. Biophys. Res. Commun. 295 (2005) 937-944 | en |
dc.identifier.issn | 0006-291X (Print) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=12127985 | - |
dc.identifier.uri | https://hdl.handle.net/10371/12128 | - |
dc.description.abstract | The mechanism of TNF-alpha-mediated chondrocyte apoptosis in human articular cartilage was investigated. First passage OA chondrocytes were treated with actinomycin D or MG132 in combination with TNF-alpha to facilitate cell death. The patterns of apoptosis-related proteins, NF-kappaB activation, and IkappaB degradation were analyzed. Cell death was increased by 0.2 microg/ml of actinomycin D or 20 microM MG132 in combination with TNF-alpha. Apoptosis potentiated by MG132 was more effectively inhibited by caspase inhibitors than that by actinomycin D. MG132 or actinomycin D both led to a significant increase in p53, but the expressions of the p53 response proteins increased only in MG132 treated chondrocytes. TNF-alpha induced chondrocyte IkappaB phosphorylation was unaffected by either MG132 or actinomycin D. MG132, but not actinomycin D, inhibited the chondrocyte IkappaB degradation induced by TNF-alpha and NF-kappaB activation. Our results suggest that MG132 and actinomycin D exert different influences upon TNF-alpha-mediated chondrocyte apoptotic signaling. | en |
dc.language.iso | en | en |
dc.publisher | Elsevier | en |
dc.subject | Annexin A5/pharmacology | en |
dc.subject | Blotting, Western | en |
dc.subject | Caspases/antagonists & inhibitors | en |
dc.subject | Cell Nucleus/metabolism | en |
dc.subject | Chondrocytes/*metabolism/pathology | en |
dc.subject | Cysteine Proteinase Inhibitors/pharmacology | en |
dc.subject | Dactinomycin/metabolism/*pharmacology | en |
dc.subject | Flow Cytometry | en |
dc.subject | I-kappa B Proteins/metabolism | en |
dc.subject | Leupeptins/*pharmacology | en |
dc.subject | NF-kappa B/metabolism | en |
dc.subject | Protein Synthesis Inhibitors/pharmacology | en |
dc.subject | Recombinant Proteins/metabolism | en |
dc.subject | Signal Transduction | en |
dc.subject | Time Factors | en |
dc.subject | Tumor Necrosis Factor-alpha/*metabolism | en |
dc.subject | Tumor Suppressor Protein p53/metabolism | en |
dc.subject | Apoptosis | - |
dc.title | TNF-alpha-mediated apoptosis in chondrocytes sensitized by MG132 or actinomycin D | en |
dc.type | Article | en |
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