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Interface between Biomolecules and Electrical Nanostructures : 바이오분자와 전기적 나노구조의 계면에 관한 연구

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dc.contributor.advisor홍승훈-
dc.contributor.author박주훈-
dc.date.accessioned2017-07-14T00:57:33Z-
dc.date.available2017-07-14T00:57:33Z-
dc.date.issued2014-02-
dc.identifier.other000000016732-
dc.identifier.urihttps://hdl.handle.net/10371/121515-
dc.description학위논문 (박사)-- 서울대학교 대학원 : 물리·천문학부(물리학전공), 2014. 2. 홍승훈.-
dc.description.abstractRecently, nanostructures have been extensively explored to investigate the interaction between biological molecules and nanomaterial. Since the dimension of the nanostructures is in accordance with the dimension of sub-cellular molecules, such as proteins, the nanostructures directly affect the functions and structures of the proteins and are easily combined with biological molecules. Among the nanostructures, nanowires (NWs) and nanotubes (NTs) have drawn much attention for the biological applications, because NWs and NTs can provide additional mechanical, optical, and electrical advantages for the nanostructure and biomolecule composites. However, it has not been extensively studied to visualize the interaction between nanostructures and biomolecules, optically and electrically.
Here, we first demonstrated the sub-diffraction limit imaging of individual inorganic NWs under cells for the analysis of the interactions between the NWs and the focal adhesions of the cells. We successfully demonstrated the sub-diffraction limit optical imaging of sub-100 nm diameter NWs and developed an analysis method to extract the cross-sectional dimensions of the imaged NWs. Also, since this method is compatible with other fluorescence based methods and biological environments, we could image NWs as well as the focal adhesions of cells grown on the NWs to analyze the effect of NWs on the cell growth. Our works show that super resolution fluorescence imaging methods, which have been mostly utilized for biological imaging until now, can be a useful strategy for the imaging and the precise analysis of inorganic nanostructures with significant advantages over other imaging methods.
Secondly, we demonstrated an olfactory-nanovesicle-fused carbon-nanotube-transistor biosensor (OCB) that mimics the responses of a canine nose for the monitoring of the response of olfactory receptors (cfOR5269) to hexanal, an indicator of the oxidation of food. OCBs allowed us to monitor the response of cfOR5269 to hexanal in real-time. Significantly, we demonstrated the detection of hexanal with an excellent selectivity capable of discriminating hexanal from analogous compounds such as pentanal, heptanal, and octanal. Furthermore, we successfully detected hexanal in spoiled milk without any pretreatment processes. Considering these results, our sensor platform should offer a new method for the assessment of food quality and contribute to the development of portable sensing devices.
Finally, we demonstrated a peptide receptor-based bioelectronic nose (PRBN) that can determine the quality of seafood in real-time through measuring the amount of trimethylamine (TMA) generated from spoiled seafood. The PRBN allowed us to sensitively and selectively detect TMA in real-time at concentrations as low as 10 fM. Also, we were able to not only determine the quality of three kinds of seafood (oyster, shrimp, and lobster), but were also able to distinguish spoiled seafood from other types of spoiled foods without any pretreatment processes.
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dc.description.tableofcontentsTable of contents 6
List of figures 8
Chapter 1 Introduction 10
1.1 Previous Studies on Biomolecule-Nanostructure Interface 11
1.2 Motivation 13
1.3 References 15
Chapter 2 Sub-Diffraction Limit Imaging of Inorganic Nanowire Networks Interfacing Cells 16
2.1 Introduction 17
2.2 Experimental Procedure 20
2.3 STORM Imaging of SnO2 NWs 23
2.4 Comparison of the Resolution of AFM, SEM and STORM 28
2.5 High Resolution Imaging of SnO2 Nanowires Interfacing a Cell 31
2.6 Summary 35
2.7 References 36
Chapter 3 Electrical Monitoring of the Activity of Olfactory Vesicles 39
3.1 Introduction 40
3.2 Preparation of Nanovesicle-CNT Hybrid Structure 42
3.3 Confirmation of the Functionality of cfOR5269 in Nanovesicles 47
3.4 Electrical Monitoring of Olfactory Nanovesicle Activity 49
3.5 Monitoring of the Response of Olfactory Nanovesicles to Spoiled Milk 55
3.6 Summary 56
3.7 References 57
Chapter 4 Electrical Monitoring of the Binding between Odorants and Olfactory Peptide Receptors 60
4.1 Introduction 61
4.2 Preparation of Peptide Receptor based Bioelectronic Noses 64
4.3 Characterization of the Immobilization of Peptides on CNTs 67
4-4. Electrical Monitoring of the Binding between TMA and Peptide Receptors 69
4.5 Electrical Monitoring of Response of Peptide Receptors to Seafood Samples 72
4.6 Summary 78
4.7 References 79
Chapter 5 Conclusions 82
Chapter 6 Abstract in Korean 85
Acknowledgement 88
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dc.formatapplication/pdf-
dc.format.extent2297564 bytes-
dc.format.mediumapplication/pdf-
dc.language.isoen-
dc.publisher서울대학교 대학원-
dc.subjectNanowire-
dc.subjectCarbon Nanotube-
dc.subjectCell-
dc.subjectProtein-
dc.subjectField Effect Transistor-
dc.subject.ddc523-
dc.titleInterface between Biomolecules and Electrical Nanostructures-
dc.title.alternative바이오분자와 전기적 나노구조의 계면에 관한 연구-
dc.typeThesis-
dc.contributor.AlternativeAuthorJuhun Park-
dc.description.degreeDoctor-
dc.citation.pages89-
dc.contributor.affiliation자연과학대학 물리·천문학부(물리학전공)-
dc.date.awarded2014-02-
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