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Pathogenesis and Immune Reaction in C57BL/6 and CBA/N Mice Infected with Clonorchis sinensis : 간흡충에 감염된 C57BL/6 마우스와 CBA/N 마우스 모델에서 병리학적 및 면역반응 연구
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 홍성태 | - |
dc.contributor.author | JIN YAN | - |
dc.date.accessioned | 2017-07-14T01:22:05Z | - |
dc.date.available | 2017-07-14T01:22:05Z | - |
dc.date.issued | 2013-02 | - |
dc.identifier.other | 000000008853 | - |
dc.identifier.uri | https://hdl.handle.net/10371/121882 | - |
dc.description | 학위논문 (박사)-- 서울대학교 대학원 : 의학과 기생충학 전공, 2013. 2. 홍성태. | - |
dc.description.abstract | To develop a mouse model for the early immune responses in clonorchiasis, the CBA/N mouse, in which Th1 and Th17 responses dominate, and the C57BL/6, in which the Th2 response is dominant were examined. Two different strains of mice were infected with Clonorchis sinensis metacercariae and analyzed 2, 4, and 8 weeks later. CBA/N mice infected with C. sinensis developed more severe gross and histopathological changes than C57BL/6 mice, including more pronounced inflammatory cell infiltration and hepatocyte necrosis in CBA/N mice, especially at 2 weeks post-infection. Additionally, serum biomarkers of liver injury (ALT and AST) were increased significantly in CBA/N mice after C. sinensis infection, compared with those in infected C57BL/6 mice. Proliferation of splenocytes and cytokine production of the splenocytes was analyzed after stimulation with crude C. sinensis antigen. The splenocytes from CBA/N mice were highly proliferative and the IL-17 level was increased. Additionally, an increased IL-4 level was sustained until 8 weeks post-infection in CBA/N mice. The serum levels of C. sinensis-specific IgG1 and IgG2a were increased in CBA/N mice, whereas IgE was increased in C57BL/6 mice. To directly ascertain the role of IL-17 in the development of C. sinensis-induced liver inflammation, IL-17-neutralizing mAb were treated after infected with C. sinensis. Because IL-17 production was increased from 2 weeks p.i., infected C57BL/6 and CBA/N mice were systemically administrated with anti-IL-17 or rat IgG2a isotype control and the mice were sacrificed on 2 weeks p.i. Anti-IL-17 mAb, but not the control mAb, significantly improved the liver histopathological appearance. Accordingly, anti-IL-17 mAb-treated mice displayed attenuated liver injury, as indicated by considerably decreased levels of serum ALT (from 284 ± 162.8 to 70 ± 16.4) and AST (from 215 ± 65.5 to 121 ± 18.6) in CBA/N mice. Intrahepatic lymphocytes and splenocytes were isolated from each mouse and counted. Their numbers were significantly decreased from C. sinensis-infected mice treated with IL-17-neutralizing mAb than the positive control both in CBA/N mice. In conclusion, the CBA/N mouse is a good animal model to study early immune response after infection with C. sinensis. The infected CBA/N mice stimulate differentiation of pathogenic Th17 cells as well as to produce humoral immunity by B cells. IL-17 is playing a key role for development of severe immunopathology of the liver in early stage of clonorchiasis. | - |
dc.description.tableofcontents | CONTENTS
Abstract i Contents iii List of Figures iv List of Tables vi List of Abbreviations vii Introduction 1 Material and Methods 7 Results 14 Discussion 38 References 44 Abstract in Korean 54 Acknowledgement 56 | - |
dc.format | application/pdf | - |
dc.format.extent | 2152653 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | Clonorchiasis | - |
dc.subject | Mouse | - |
dc.subject | CBA/N | - |
dc.subject | C57BL/6 | - |
dc.subject | Cytokines | - |
dc.subject | IL-17 | - |
dc.subject | Immunoglobulin | - |
dc.subject.ddc | 610 | - |
dc.title | Pathogenesis and Immune Reaction in C57BL/6 and CBA/N Mice Infected with Clonorchis sinensis | - |
dc.title.alternative | 간흡충에 감염된 C57BL/6 마우스와 CBA/N 마우스 모델에서 병리학적 및 면역반응 연구 | - |
dc.type | Thesis | - |
dc.contributor.AlternativeAuthor | 김 연 | - |
dc.description.degree | Doctor | - |
dc.citation.pages | 56 | - |
dc.contributor.affiliation | 의과대학 의학과 | - |
dc.date.awarded | 2013-02 | - |
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