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Role of AMPK in cardioprotection by leptin-preconditioning : 렙틴 전처치에 의한 심장보호기전에서 AMPK의 역할
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 호원경 | - |
dc.contributor.author | 김기석 | - |
dc.date.accessioned | 2017-07-14T01:25:43Z | - |
dc.date.available | 2017-07-14T01:25:43Z | - |
dc.date.issued | 2014-02 | - |
dc.identifier.other | 000000016550 | - |
dc.identifier.uri | https://hdl.handle.net/10371/121944 | - |
dc.description | 학위논문 (박사)-- 서울대학교 대학원 : 의학과, 2014. 2. 호원경. | - |
dc.description.abstract | Introduction: Leptin, a product of the ob gene, is a 16-kDa peptide synthesized primarily by white adipose tissue. It is also produced in the heart, suggesting that it has a cardioprotective effect. AMP-activated protein kinase (AMPK) is a serine/threonine protein kinase that plays a key role in cardioprotection in the abnormal heart. However, it is not clear whether AMPK-mediated cardioprotection influences the effects of leptin in cardiac muscle. Here, I investigated the mechanism of cardioprotection in leptin preconditioning and the relationship between leptin preconditioning-induced cardioprotection and the regulation of AMPK.
Methods: A rat ischemia model was constructed using left anterior descending coronary artery occlusion (CAO). Infarct size was measured to evaluate the cardioprotective effect. A rat cardiac myoblast cell line (H9c2) was used to investigate the cardioprotective mechanism of leptin. Results: Leptin decreased myocardial infarct size in rat cardiac ischemic reperfusion model. The effect of leptin was abolished in combination with compound C, an AMPK inhibitor or NAC, a ROS scavenger. Leptin phosphorylated AMPK dose- and time-dependently in H9c2 cells. Leptin also induced generation of reactive oxygen species (ROS) dose- and time-dependently in H9c2 cells. AMPK phosphorylation by leptin was abolished in combination with NAC, however, leptin-induced ROS generation was not affected by compound C. Therefore, Leptin induced generation of ROS followed by activation of AMPK. Activated AMPK decreased cardiac ischemic damage. Conclusions: AMPK activation plays a key role in leptin preconditioning-induced cardioprotection. Leptin-induced ROS generation results in AMPK activation. | - |
dc.description.tableofcontents | Contents i
List of tables and figures ii List of abbreviations iii Introduction 1 Material and Methods 5 Results 12 Discussion 43 References 49 Abstract in Korean 58 | - |
dc.format | application/pdf | - |
dc.format.extent | 1809554 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject.ddc | 610 | - |
dc.title | Role of AMPK in cardioprotection by leptin-preconditioning | - |
dc.title.alternative | 렙틴 전처치에 의한 심장보호기전에서 AMPK의 역할 | - |
dc.type | Thesis | - |
dc.contributor.AlternativeAuthor | Ki-Suk Kim | - |
dc.description.degree | Doctor | - |
dc.citation.pages | iv, 59 | - |
dc.contributor.affiliation | 의과대학 의학과 | - |
dc.date.awarded | 2014-02 | - |
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