S-Space College of Medicine/School of Medicine (의과대학/대학원) Dept. of Medicine (의학과) Theses (Ph.D. / Sc.D._의학과)
Effects of polyunsaturated fatty acids on hair growth
고도불포화 지방산이 모발 성장에 미치는 영향
- 의과대학 의학과
- Issue Date
- 서울대학교 대학원
- hair growth PUFA
- 학위논문 (박사)-- 서울대학교 대학원 : 의학과 피부과학전공, 2015. 8. 권오상.
- Hair loss is a common problem but the treatments for the various forms of hair loss showed limited success yet. Polyunsaturated fatty acids (PUFAs) are long chain fatty acids with more than one C?C double bond in their backbones. From the ancient times, animal and plant oils, which have rich polyunsaturated fatty acids, have been used as a traditional remedy for treating hair loss in the several countries. Arachidonic acid (AA) and Eicosapentaenoic acid (EPA) are omega-6 and omega-3 fatty acid each and exert their effects through changes in membrane phospholipids or the production of signaling molecules such as eicosanoids.
The purpose of this study is evaluate effects of polyunsaturated fatty acids on hair growth, and find out the possible mechanism of hair growth.
Therefore, we tested whether AA and EPA had effects on anagen induction or anagen prolongation using C57BL/6 mice. We also investigated the effect of AA on human dermal papilla cells (hDPCs) and in ex vivo organ culture model.
Anagen induction was tested with AA and EPA of 2% and AA plus EPA groups mixed with ratio of 1:1, 1:3, and 3:1, respectively. The regeneration of new hair coat was more prominently observed in 2% AA and AA plus EPA 3:1 treated group and moderately accelerated in 2% EPA and AA plus EPA 1:1, 1:3 group compared with control group. Furthermore, 2% AA and AA plus EPA 3:1 group markedly prolonged the anagen hair growth in an in vivo model of anagen prolongation induced by depilation.
AA significantly promoted elongation with increased proliferation of matrix keratinocytes ex vivo culture.
We found that AA enhanced the viability of hDPCs also promoted the expression of several factors that are known to promote hair growth, including fibroblast growth factor FGF-7, FGF-10 and hepatocyte growth factor.
Western blotting revealed that AA in hDPCs led to phosphorylation various transcription factors (ERK, CREB, and AKT) and increased the expression of Bcl2. These results suggest that PUFA plays role in hair growth. AA and EPA, both are polyunsaturated fatty acid as a broad composition in plant and animal products, could be a potential candidate for the promotion of hair growth.