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The role of Helicobacter pylori on gastric carcinogenesis through epithelial-mesenchymal transition : 헬리코박터 파일로리균이 상피-간엽전환을 통해 위암발생에 미치는 역할
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 김나영 | - |
dc.contributor.author | Yoon Jin Choi | - |
dc.date.accessioned | 2017-07-14T01:34:37Z | - |
dc.date.available | 2017-07-14T01:34:37Z | - |
dc.date.issued | 2016-02 | - |
dc.identifier.other | 000000132786 | - |
dc.identifier.uri | https://hdl.handle.net/10371/122119 | - |
dc.description | 학위논문 (박사)-- 서울대학교 대학원 : 의과대학 의학과 중개의학전공, 2016. 2. 김나영. | - |
dc.description.abstract | We know little concerning the expression of transforming growth factor-β1 (TGF-β1) and TGF-β1-induced epithelial-mesenchymal transition (EMT) markers in gastric mucosa and their changes after eradication of Helicobacter pylori infection have not yet been clarified. In the present study, we compared the time course of mRNA expression of TGF-β1 and 5 EMT markers (Twist, Snail, Slug, vimentin, and E-cadherin) in 111 controls, 55 patients with gastric dysplasia, and 71 patients with early gastric cancer, following eradication of H. pylori. mRNA levels in noncancerous gastric mucosa were measured using qRT-PCR and the histologic findings of gastric mucosa were compared before and after eradication. The average duration of follow-up was 46.7 months (6.0–112.4). The levels of TGF-β1, Twist, Snail, Slug, and vimentin mRNA, in addition to levels of CD44 and Leucine-rich repeat-containing G-protein coupled receptor 5 (Lgr5) detected by immunohistochemistry, showed all up-regulation in patients with dysplasia or early gastric cancer compared with controls (P < 0.05) | - |
dc.description.abstract | moreover, the mRNA levels of E-cadherin, an epithelial marker, were decreased in these patients compared with the control group (P < 0.001). Eradication of H. pylori reduced the expression of TGF-β1, Twist, Snail, Slug and vimentin mRNA (P-value for slope < 0.001), as well as the immunohistochemical expression of CD44 (P = 0.014), whereas it enhanced the expression of E-cadherin (P-value for slope < 0.05). Thus, H. pylori infection may trigger the TGF-β1-induced EMT pathway and the emergence of gastric cancer stem cells. Its eradication may prevent the carcinogenesis of gastric cancer by inhibiting these 2 pathways. | - |
dc.description.tableofcontents | 1 Introduction 1
2 Materials and Methods 5 2.1 Patient Selection 5 2.2 H. pylori testing and histology 6 2.3 Real-time quantitative polymerase chain reaction 7 2.4 Immunohistochemistry 8 2.5 Statistical analysis 9 3 Results 10 3.1 Demographic characteristics 10 3.2 Expression of mRNAs encoding EMT-markers and CD44, and IHC analysis in H. pylori-positive subjects 11 3.3 Association between genes encoding EMT markers and CD44 expression 12 3.4 Expression of TGF-β1 and EMT markers between the H. pylori-positive and H. pylori-negative groups 13 3.5 Effects of H. pylori eradication on histologic grade in gastric mucosa 15 3.6 Effects of H. pylori eradication on the expression of TGF-β1, EMT markers, and CD44 15 3.7 Relationship between TGF-β1/EMT markers and intestinal metaplasia 17 3.8 Association between LGR5 immunoreactivity and gastric carcinogenesis 18 3.9 Effects of H. pylori eradication on the expression of LGR5 19 3.10 Association between expression of LGR5 and CD44 20 3.11 Expression of LGR5 IHC according to the location of gastric mucosa and intestinal metaplasia 20 4 Discussion 21 5 References 29 초록 37 Table and Figure 39 | - |
dc.format | application/pdf | - |
dc.format.extent | 865899 bytes | - |
dc.format.medium | application/pdf | - |
dc.language.iso | en | - |
dc.publisher | 서울대학교 대학원 | - |
dc.subject | Helicobacter pylori | - |
dc.subject | epithelial-mesenchymal transition | - |
dc.subject | cancer stem cells | - |
dc.subject.ddc | 610 | - |
dc.title | The role of Helicobacter pylori on gastric carcinogenesis through epithelial-mesenchymal transition | - |
dc.title.alternative | 헬리코박터 파일로리균이 상피-간엽전환을 통해 위암발생에 미치는 역할 | - |
dc.type | Thesis | - |
dc.contributor.AlternativeAuthor | 최윤진 | - |
dc.description.degree | Doctor | - |
dc.citation.pages | 63 | - |
dc.contributor.affiliation | 의과대학 의학과 | - |
dc.date.awarded | 2016-02 | - |
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