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Iontophoretic transport of poly(lactic-co-glycolic acid) nanoparticles across in vitro rabbit cornea : 이온영동 방법을 통한 poly(lactic-co-glycolic acid) 나노 입자의 토끼 각막으로의 전달양상

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dc.contributor.advisor최영빈-
dc.contributor.author이재연-
dc.date.accessioned2017-07-14T02:24:20Z-
dc.date.available2017-07-14T02:24:20Z-
dc.date.issued2017-02-
dc.identifier.other000000142051-
dc.identifier.urihttps://hdl.handle.net/10371/122470-
dc.description학위논문 (석사)-- 서울대학교 대학원 : 바이오엔지니어링전공, 2017. 2. 최영빈.-
dc.description.abstractIontophoresis is a non-invasive technique used to transport substances of interest across tissues and this has drawn interest in ophthalmic fields to enhance delivery efficiency of topically administered drugs. Thus, there have been numerous trials to deliver small molecules across cornea into the eye using iontophoresis-
dc.description.abstracthowever, few were reported to transport the nanoparticles. The purpose of this study was, therefore, to profile an iontophoretic transport of the nanoparticles made of a biodegradable polymer, poly(lactic-co-glycolic acid) (PLGA), where the various conditions of iontophoresis were applied via the cornea of in vitro rabbit eyes. Also, iontophoresis was conducted on the simulated eyelid to minimize the potential problems caused by direct contact of an electrode to the sensitive eye surface. For this, the tablet formulation of fluorescence-tagged PLGA nanoparticles was applied onto the cornea, where the factors, such as the size of nanoparticles, amplitude of electric current and time for iontophoresis application, were varied. After the iontophoretic application of the nanoparticles, the fluorescence intensity of each of the cross-section layers of the cornea was observed with confocal fluorescence microscopy to assess the distribution of PLGA nanoparticles. The results show that there are significant differences in the delivered amount of PLGA nanoparticles into cornea according to the size of PLGA nanoparticles and time of formulation applications. Importantly, the particle size was observed to be one the most crucial factors in determining the transport of PLGA nanoparticles across cornea. In this study, however, although the intensity of electric current was varied at 0 mA to 0.5 mA, 1 mA and 2 mA, there were no statistically significant differences among the delivered amounts of PLGA nanoparticles. Under the iontophoresis conditions employed in this study, it appeared that the electric field was not properly focused on the cornea and the surface charge of the nanoparticles was not very high (~ 2 mV). Therefore, transport of PLGA nanoparticles across in vitro cornea was mostly mediated by particle diffusion.-
dc.description.tableofcontents1. Introduction 1
1.1. Study Background 1
1.2. Purpose of Research 3
2. Materials and Methods 5
2.1. Materials 5
2.2. Preparation of tablet formulation of PLGA nanoparticles 5
2.3. Characterization of PLGA nanoparticles 6
2.4. In vitro experimental setup 6
2.5. Semi-quantification of delivered PLGA nanoparticles 7
3. Results 11
3.1. PLGA nanoparticles characterization 11
3.2. Effects of electric current intensity 16
3.3. Effects of iontophoresis application time 26
3.4. Effects of particles size 37
4. Discussion 41
5. Conclusion 44
Bibliography 46
국문초록 50
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dc.formatapplication/pdf-
dc.format.extent2401894 bytes-
dc.format.mediumapplication/pdf-
dc.language.isoen-
dc.publisher서울대학교 대학원-
dc.subjectcornea-
dc.subjectdiffusion-
dc.subjectin vitro-
dc.subjectiontophoresis-
dc.subjectPLGA nanoparticles-
dc.subject.ddc660-
dc.titleIontophoretic transport of poly(lactic-co-glycolic acid) nanoparticles across in vitro rabbit cornea-
dc.title.alternative이온영동 방법을 통한 poly(lactic-co-glycolic acid) 나노 입자의 토끼 각막으로의 전달양상-
dc.typeThesis-
dc.contributor.AlternativeAuthorJae Yeon Lee-
dc.description.degreeMaster-
dc.citation.pages52-
dc.contributor.affiliation공과대학 협동과정 바이오엔지니어링전공-
dc.date.awarded2017-02-
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