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Transcriptome analysis of small brown planthopper, Laodelphax striatellus carrying Rice Stripe Virus : 벼 줄무늬잎마름병을 매개하는 애멸구의 전사체 분석

DC Field Value Language
dc.contributor.advisor제연호-
dc.contributor.author이주현-
dc.date.accessioned2017-07-14T06:38:28Z-
dc.date.available2017-07-14T06:38:28Z-
dc.date.issued2013-02-
dc.identifier.other000000009150-
dc.identifier.urihttps://hdl.handle.net/10371/125796-
dc.description학위논문 (석사)-- 서울대학교 대학원 : 농생명공학부, 2013. 2. 제연호.-
dc.description.abstractRice stripe virus (RSV), the type member of the genus Tenuivirus which causes rice stripe disease, transmits by the feeding behavior of small brown planthopper (SBPH), Laodelphax striatellus. Because RSV replicates in SBPH persistently and SBPH has sap-sucking feeding behavior with its piercing-sucking mouthpart in the phloem, SBPH is one of the most notorious vectors of RSV. So far, most considerations have been focused on the protection of the rice from RSV and/or the SBPH rather than the interactions among the rice, RSV and SBPH. Hence, to investigate the interactions between the virus and vector insect, total RNA was extracted from RSV-viruliferous SBPH (RVLS) and non-viruliferous SBPH (NVLS) adults to construct expressed sequence tag databases for comparative transcriptome analysis. Over 30 million bases were sequenced by 454 pyrosequencing to construct 1,538 and 953 of isotigs of SBPH from the mRNA of RVLS and NVLS, respectively. The gene ontology (GO) analysis demonstrated that both transcriptomes of RVLS and NVLS have similar GO structure, however, the gene expression pattern analysis revealed that 108 genes were up-regulated and 28 genes were down-regulated significantly in the RVLS. These RSV-dependently regulated genes of SBPH possibly have important roles in the physiology and behavior of SBPH, transmission of RSV, and RSV and SBPH interaction.
Also, two kinds of picorna-like viruses were newly discovered from the EST libraries of each NVLS and RVLS, Which were named LsPV-1 and LsPV-2, respectively. The sequence analysis result revealed that LsPV-1 was a Korean isolate of Himetobi-P virus (HiPV) which belongs to dicistorovidae while the LsPV-2 genome structure resembled that of iflaviridae. A phylogenetic analysis of LsPV-2 RNA dependent RNA polymerase sequence showed that honeybee deformed wing virus was the most closely related virus. Interestingly, RSV and HiPV seem not to superinfect SBPH which already carrying the other virus, therefore, the incompatibility between RSV and HiPV might give us a clue to understand the viral microflora and the mechanism of persistency in SBPH. Also, The HiPV and LsPV-2 were incompatible each other in SBPH, suggesting that theses tow picorna-like viruses may have important functions in transmission of the RSV.
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dc.description.tableofcontentsABSTRACT
TABLE OF CONTENTS
LIST OF TABLES
LIST OF FIGURES
INTRODUCTION
LITERATURE REVIEW
1.Laodelphax striatellus
2.General characteristics of Rice stripe virus
3.Next Generation Sequencing
4.Picorna-like virus
MATERIALS AND METHODS
1)RSV-viruliferous and non-viruliferous L. striatellus
2)Total RNA extraction and RT-PCR
3)Transciptome sequencing and EST library construction
4)Gene ontology analysis and Comparison of gene expression
5)Analysis of relative transcription levels by qPCR
6)cDNA sequencing of two picorna-like viruses
7)Sequence and phylogenetic analysis
RESULTS
1.EST Analysis of Small Brown Planthopper, Laodelphax striatellus Carrying Rice Stripe Virus
1)RT-PCR for confirmation of RVLS or NVLS
2)Transciptome sequencing results and EST library analysis
3)Gene ontology analysis
4)Comparison of gene expression
5)Comparison of transcriptom profiles between RNLS and NVLS using qPCR
6)Rice stripe virus transcripts in RVLS
2.Identification and Genome Sequence of Two Picorna-like Viruses in Laodelphax striatellus
1)Sequence analysis of the two picorna-like viruses genome
2)Predicted amino acid sequence and phylogenetic analysis
DISCUSSION
LITERATURE CITED
ABSTRACT IN KOREAN
ACKNOWLEDGEMENTS
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dc.formatapplication/pdf-
dc.format.extent891799 bytes-
dc.format.mediumapplication/pdf-
dc.language.isoen-
dc.publisher서울대학교 대학원-
dc.subject.ddc630-
dc.titleTranscriptome analysis of small brown planthopper, Laodelphax striatellus carrying Rice Stripe Virus-
dc.title.alternative벼 줄무늬잎마름병을 매개하는 애멸구의 전사체 분석-
dc.typeThesis-
dc.description.degreeMaster-
dc.citation.pagesvi,44-
dc.contributor.affiliation농업생명과학대학 농생명공학부-
dc.date.awarded2013-02-
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